Periodontitis may induce gut microbiota dysbiosis via salivary microbiota.
10.1038/s41368-022-00183-3
- Author:
Jun BAO
1
;
Lili LI
1
;
Yangheng ZHANG
1
;
Min WANG
1
;
Faming CHEN
2
;
Shaohua GE
3
;
Bin CHEN
4
;
Fuhua YAN
5
Author Information
1. Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, No.30 Zhongyang Road, Nanjing, China.
2. State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi'an, China.
3. Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
4. Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, No.30 Zhongyang Road, Nanjing, China. 361734128@qq.com.
5. Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, No.30 Zhongyang Road, Nanjing, China. yanfh@nju.edu.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Dysbiosis;
Gastrointestinal Microbiome;
Humans;
Mice;
Mice, Inbred C57BL;
Microbiota;
Periodontitis;
RNA, Ribosomal, 16S/metabolism*
- From:
International Journal of Oral Science
2022;14(1):32-32
- CountryChina
- Language:English
-
Abstract:
The aim of this study was to identify whether periodontitis induces gut microbiota dysbiosis via invasion by salivary microbes. First, faecal and salivary samples were collected from periodontally healthy participants (PH group, n = 16) and patients with severe periodontitis (SP group, n = 21) and analysed by 16S ribosomal RNA sequencing. Significant differences were observed in both the faecal and salivary microbiota between the PH and SP groups. Notably, more saliva-sourced microbes were observed in the faecal samples of the SP group. Then, the remaining salivary microbes were transplanted into C57BL6/J mice (the C-PH group and the C-SP group), and it was found that the composition of the gut microbiota of the C-SP group was significantly different from that of the C-PH group, with Porphyromonadaceae and Fusobacterium being significantly enriched in the C-SP group. In the colon, the C-SP group showed significantly reduced crypt depth and zonula occludens-1 expression. The mRNA expression levels of pro-inflammatory cytokines, chemokines and tight junction proteins were significantly higher in the C-SP group. To further investigate whether salivary bacteria could persist in the intestine, the salivary microbiota was stained with carboxyfluorescein diacetate succinimidyl ester and transplanted into mice. We found that salivary microbes from both the PH group and the SP group could persist in the gut for at least 24 h. Thus, our data demonstrate that periodontitis may induce gut microbiota dysbiosis through the influx of salivary microbes.
