A case of severe hookworm infection.
10.11817/j.issn.1672-7347.2022.210593
- Author:
Linghui MA
1
;
Dibo NOUHOUM
2
;
Wanni WANG
3
;
Zhishan ZHANG
3
;
Xunya HOU
2
;
Xinling YU
2
;
Weicheng DENG
2
;
Jie GUAN
4
;
Shuaiqin HUANG
5
;
Xiang WU
6
Author Information
1. Department of Laboratorial Examination, Quanzhou First Affiliated Hospital of Fujian Medical University, Quanzhou Fujian 362000. 462467826@qq.com.
2. Department of Prevention and Control, Hunan Institute of Schistosomiasis Control, Yueyang Hunan 414021.
3. Department of Laboratorial Examination, Quanzhou First Affiliated Hospital of Fujian Medical University, Quanzhou Fujian 362000.
4. Department of Pathology, Hunan Aerospace Hospital, Changsha 410006.
5. Key Laboratory of Immunization and Transmission Control of Schistosomiasis in Hunan Province, Yueyang Hunan 414125. huangshuaiqin@xmu.edu.cn.
6. Key Laboratory of Immunization and Transmission Control of Schistosomiasis in Hunan Province, Yueyang Hunan 414125. wxspring@126.com.
- Publication Type:Journal Article
- Keywords:
hookworm;
semi-nested RT-PCR;
severe infection;
species identification
- MeSH:
Ancylostomatoidea/genetics*;
Animals;
Feces;
Hookworm Infections/diagnosis*;
Humans;
Necator americanus/genetics*;
Polymerase Chain Reaction
- From:
Journal of Central South University(Medical Sciences)
2022;47(6):814-822
- CountryChina
- Language:English
-
Abstract:
With the improvement of sanitation, the infection rate of hookworm is greatly reduced and the severe infected case is rarely reported. Combined morphological and molecular biological examinations, a severe hookworm infection patient was diagnosed in Department of Laboratorial Examination, Quanzhou First Affiliated Hospital of Fujian Medical University. The morphological methods such as direct fecal smear microscopy, saturated brine flotation and hookworm larvae culture methods were used to identify the eggs and larvae from stool samples of the patient. There were a large number of hookworm eggs in patient's stool samples, and the average count was 60 840 per gram by modified Kato method, which belonged to severe hookworm infection. Meanwhile, to distinguish the hookworm species, the semi-nested RT-PCR assay was employed to detect hookworm internal transcribed spacer series from eggs in patient's stool samples, and the result showed that the hookworm species was confirmed to be Necator americanus.
