Aspirin inhibits the growth of hypertrophic scar in rabbit ears via regulating Wnt/β-catenin signal pathway.
10.11817/j.issn.1672-7347.2022.220038
- Author:
Zhihu LIN
1
;
Xiao HAN
2
;
Mengyao ZHANG
2
;
Jiaqin XU
3
;
Haihong LI
4
;
Jianda ZHOU
2
;
Huiqing XIE
5
Author Information
1. Department of Burn and Plastic Surgery, Third Xiangya Hospital, Central South University, Changsha 410013. swiftxiaohu@126.com.
2. Department of Burn and Plastic Surgery, Third Xiangya Hospital, Central South University, Changsha 410013.
3. Department of Burn and Skin Repair Surgery, Hainan General Hospital, Haikou 570311.
4. Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan Hubei 442000.
5. Department of Rehabilitation Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China. xiaoxiang0733@126.com.
- Publication Type:Journal Article
- Keywords:
Wnt/β-catenin signal pathway;
aspirin;
hypertrophic scar;
triamcinolone acetonide
- MeSH:
Animals;
Anti-Inflammatory Agents/therapeutic use*;
Aspirin/therapeutic use*;
Cicatrix, Hypertrophic/pathology*;
Collagen;
Rabbits;
Signal Transduction;
Triamcinolone Acetonide/therapeutic use*;
beta Catenin/metabolism*
- From:
Journal of Central South University(Medical Sciences)
2022;47(6):698-706
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Steroidal anti-inflammatory drugs have certain side effects in the treatment of hypertrophic scar, and the scar recurrence is easy after withdrawal of steroid anti-inflammatory drugs. Finding reliable alternative drugs is an effective means to improve this defect. Aspirin, a traditional non-steroidal anti-inflammatory drug, is safe for topical use and has anti-inflammatory effects similar to those of steroidal anti-inflammatory drugs, which may have similar effects on the treatment of hypertrophic scar. This study aims to investigate the inhibitory effect of aspirin on the proliferation of hypertrophic scar in rabbit ears and the underlying mechanism.
METHODS:The rabbit ear hypertrophic scar models were prepared. The rabbits were randomly divided into a normal skin group (group A), a blank control group (group B), a 0.9% NaCl group (group C), a 0.2% aspirin group (group D), a 0.5% aspirin group (group E), a 2% aspirin group (group F), and a triamcinolone acetonide group (group G). Macroscopic observation of hyperplasia was performed 8 weeks after local injection of the scar, followed by collecting the scar tissue samples for HE staining, Masson staining, and immunohistochemistry, respectively to assess the proliferation of fibroblasts and collagen fibers, and calculate the hypertrophic index, microvessel density, and immunohistochemical score.
RESULTS:All rabbit ear hypertrophic scar models were successfully constructed. In groups B and C, the hypertrophic scar edge was irregular, with reddish protruding epidermis, significant contracture and hard touch. In group D, E, and F, with the increase of aspirin administration concentration, the scar became thinner and gradually flat, the proliferation of fibrocytes and collagen fibers was weakened, and the hypertrophic index was gradually decreased (P<0.05). Immunohistochemistry showed that the expression of β-catenin was decreased in the group D, E and F in turn, and the immunohistochemical score was gradually decreased (P<0.05). There was no significant difference in hypertrophic index, microvessel density, and immunohistochemical score (all P>0.05).
CONCLUSIONS:Local injection of aspirin can reduce the generation of hypertrophic scar in a dose-dependent manner within a certain concentration range; aspirin inhibits the growth of hypertrophic scar in rabbit ears by inhibiting Wnt/β-catenin signal pathway; 2% aspirin and 40 mg/mL triamcinolone acetonide have similar curative efficacy on hypertrophic scar.
