Anti-inflammatory Effects of Ampelopsis Japonica Root on Contact Dermatitis in Mice.
10.1007/s11655-022-3517-0
- Author:
Yoona OH
1
;
Hongbum LEE
1
;
Beodeul YANG
1
;
Sura KIM
1
;
Hyunwoo JEONG
2
;
Hyungwoo KIM
3
Author Information
1. Division of Pharmacology, School of Korean Medicine, Pusan National University, Gyeongnam, 50612, South Korea.
2. Department of Pathology, College of Korean Medicine, Dongshin University, Jeonnam, 58245, South Korea.
3. Division of Pharmacology, School of Korean Medicine, Pusan National University, Gyeongnam, 50612, South Korea. kronos7@pusan.ac.kr.
- Publication Type:Journal Article
- Keywords:
Ampelopsis japonica;
Chinese medicine;
contact dermatitis;
dermatosis;
inflammation
- MeSH:
Ampelopsis;
Animals;
Anti-Inflammatory Agents/therapeutic use*;
Cytokines;
Dermatitis, Contact/pathology*;
Dinitrofluorobenzene/therapeutic use*;
Hyperplasia/drug therapy*;
Interleukin-6;
Mice;
Mice, Inbred BALB C;
Plant Extracts/therapeutic use*;
Tumor Necrosis Factor-alpha
- From:
Chinese journal of integrative medicine
2022;28(8):719-724
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the anti-inflammatory potential of Ampelopsis japonica on contact dermatitis (CD).
METHODS:A total of 38 Balb/c mice were divided into 5 groups by using a random number table: normal mice (n=6), CD model mice (n=8), CD mice treated with 3 or 30 mg/kg of the ethanol extract of A. japonica (EEAJ, n=8) and 7.5 mg/kg dexamethasone treated CD mice (DEX, n=8). CD was induced using topical application of 1-fluoro-2,4-dinitrofluorobenzene in mice. EEAJ and DEX were topically applied to the shaved skin of each mouse for 6 days, and the effects of EEAJ and DEX on skin lesions and color, histopathological abnormalities such as epidermal hyperplasia and immune cell infiltration, and tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) production were investigated. The effects on changes in body weights and spleen/body weight ratio were also investigated.
RESULTS:EEAJ at 30 mg/kg significantly prevented scaling, erythema and enlargement of skin weight compared to using carbon dioxide. EEAJ also prevented epithelial hyperplasia and immune cell infiltrations induced by repeated application of DNFB (P<0.01). In addition, EEAJ significantly lowered levels of TNF-α, IL-6 and MCP-1 (P<0.05 or P<0.01). The anti-inflammatory effects of EEAJ were similar to those of DEX.
CONCLUSION:A. japonica may be a new therapeutic agent with the potential to reduce or replace corticosteroids and its mechanisms are closely related to regulation of TNF-α production.
