Protective Mechanism of Electroacupuncture on Peripheral Neurotoxicity Induced by Oxaliplatin in Rats.
10.1007/s11655-022-2896-1
- Author:
Feng-Jiao WANG
1
;
She SHI
1
;
Yong-Qiang WANG
2
;
Ke WANG
3
;
Shen-Dong FAN
1
;
Ya-Nan ZHANG
3
;
Chen-Chen FENG
3
;
Zi-Yong JU
4
Author Information
1. College of Acumox and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
2. Department of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200120, China.
3. Acupuncture Anesthesia Clinical Research Institute, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
4. College of Acumox and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. juziyong@hotmail.com.
- Publication Type:Journal Article
- Keywords:
dorsal root ganglion;
electroacupuncture;
nuclear factor-erythroid-2-related factor 2;
oxaliplatin;
peripheral neuropathy
- MeSH:
Animals;
Electroacupuncture/methods*;
Hyperalgesia/therapy*;
Male;
Microcirculation;
NF-E2-Related Factor 2;
Oxaliplatin/adverse effects*;
Peripheral Nervous System Diseases/chemically induced*;
Rats;
Rats, Sprague-Dawley
- From:
Chinese journal of integrative medicine
2022;28(9):833-839
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To study the effect of electroacupuncture (EA) on oxaliplatin-induced peripheral neuropathy (OIPN) in rats.
METHODS:Male Sprague-Dawley rats were equally divided into 3 groups using a random number table: the control group, the OIPN group, and the EA (OIPN + EA) group, with 10 rats in each. The time courses of mechanical, cold sensitivity, and microcirculation blood flow intensity were determined. The morphology of the dorsal root ganglion (DRG) was observed by electron microscopic examination. The protein levels of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and the transient receptor potential (TRP) protein family in DRGs were assayed by Western blot.
RESULTS:EA treatment significantly reduced mechanical allodynia and cold allodynia in OIPN rats (P<0.01). Notably, oxaliplatin treatment resulted in impaired microcirculatory blood flow and pathomorphological defects in DRGs (P<0.01). EA treatment increased the microcirculation blood flow and attenuated the pathological changes induced by oxaliplatin (P<0.01). In addition, the expression levels of Nrf2 and HO-1 were down-regulated, and the TRP protein family was over-expressed in the DRGs of OIPN rats (P<0.01). EA increased the expression levels of Nrf2 and HO-1 and decreased the level of TRP protein family in DRG (P<0.05 or P<0.01).
CONCLUSION:EA may be a potential alternative therapy for OIPN, and its mechanism may be mainly mediated by restoring the Nrf2/HO-1 signaling pathway.
