Main Factors Influencing the Platelet Spreading.
10.19746/j.cnki.issn.1009-2137.2022.03.041
- Author:
Liu-Xia YUAN
1
;
Hong-Lei YE
1
;
Meng-Nan YANG
1
;
Xin-Xin GE
1
;
Rong YAN
1
;
Ke-Sheng DAI
2
Author Information
1. Medical College of Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, Jiangsu Province, China.
2. Medical College of Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, Jiangsu Province, China,E-mail: kdai@suda.edu.cn.
- Publication Type:Journal Article
- Keywords:
adhesion;
fibrinogen;
integrin;
spreading
- MeSH:
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid/pharmacology*;
Adenosine Diphosphate;
Animals;
Blood Platelets/physiology*;
Fibrinogen;
Humans;
Mice;
Mice, Inbred C57BL;
Platelet Adhesiveness/physiology*;
Thrombin/pharmacology*
- From:
Journal of Experimental Hematology
2022;30(3):919-923
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the main factors of platelet spreading and provide the foundation for related research.
METHODS:Platelets (2×107/ml) were draw from C57BL/6J mouse and kept at 22 ℃ for 1-2 hours. Platelets (2×107/ml) were were allowed to adhere and spread on the fibrinogen-coated slides, after staining F-actin in platelets, the platelets were observed with the confocal microscopy. The effects of different concentrations of fibrinogen (10 μg/ml, 30 μg/ml, 100 μg/ml) and kinds of agonists ［thrombin(0.01,0.05,0.1 U/ml), ADP（5,10,20 μmol/L）, U46619（0.125,0.25,0.5 μmol/L）］ on platelets were analyzed. The platelet spreading was successful if the spreading rate was higher after treated with agonists.
RESULTS:Compared to the group which coated with 10 μg/ml and 100 μg/ml fibrinogen, the platelet density is optimal when coated with 30 μg/ml fibrinogen. In addition, under the stimulation of thrombin, ADP and U46619, the spreading rate of platelets showed a certain concentration-dependent increasing.
CONCLUSION:The platelet spreading is easily influenced by various factors, the platelet spreading can be induced successfully at 0.1 U/ml thrombin, 20 μmol/L ADP and 0.5 μmol/L U46619 on the slide coated with 30 μg/ml fibrinogen.
