A Novel Lyophilized PRP-Loaded Gel Based on Chitosan and PEG with Hemostatic and Antibacterial Properties.
10.19746/j.cnki.issn.1009-2137.2022.03.034
- Author:
Ming-Zi MA
1
;
Yuan ZENG
2
;
Wei SHANG
1
;
Xiao-Xing LIANG
3
;
Lu YANG
1
;
Xiao-Long ZHONG
4
;
Li-Ping SUN
5
;
De-Qing WANG
5
;
Lei TAO
6
;
Yang YU
7
Author Information
1. Medical school of Chinese PLA, Beijing 100853, China；Department of Blood Transfusion, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
2. The Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry Tsinghua University, Beijing 100084, China.
3. Department of Blood Transfusion, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China；School of Laboratory Medicine, Bengbu Medical College, Bengbu 233030, Anhui Province, China.
4. Department of Blood Transfusion, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China；Department of Blood Transfusion, Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
5. Department of Blood Transfusion, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
6. The Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry Tsinghua University, Beijing 100084, China,E-mail:leitao@mail.tsinghua.edu.cn.
7. Department of Blood Transfusion, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China,E-mail:yuyangpla301@163.com.
- Publication Type:Journal Article
- Keywords:
chitosan;
hemostasis;
platelet-rich plasma;
polyethylene glycol
- MeSH:
Animals;
Anti-Bacterial Agents/pharmacology*;
Chitosan/pharmacology*;
Hemorrhage;
Hemostasis;
Hemostatics;
Humans;
Platelet-Rich Plasma;
Rabbits
- From:
Journal of Experimental Hematology
2022;30(3):870-876
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:A dynamic gel loaded with lyophilized platelet-rich plasma-chitosan/difunctionalized polyethylene glycol (LPRP-CP) was prepared to investigate its hemostatic antibacterial and promoting wound healing of scald wounds through in vitro and in vivo experiments.
METHODS:In this study, normal gauze/blank tablet (Ctrl), LPRP-CP, Chitosan HUCHUANG Powder(Chito P)and ChitoGauze XP PRO group (Chito G group) were set. The hemostatic effect and promoting healing effect of the four groups of materials were evaluated by establishing rabbit ear artery hemorrhage model and superficial Ⅱ° scalded model of skin on the back. The hemostatic time and bleeding amount were calculated and the gross and histological results of scald healing were observed. The antibacterial effect of the four groups of materials was evaluated by antibacterial test in vitro.
RESULTS:In the rabbit ear arterial hemorrhage model, the hemostasis of all materials was successful. The hemostatic time of Ctrl, Chito P, LPRP-CP and Chito G groups was 213.33±38.30, 118.33±24.01, 115.00±8.37 and 111.67±11.69 s, respectively. The blood loss was 1233.83±992.27, 346.67±176.00, 193.33±121.47 and 147.50±80.66 mg, respectively. Compared with Ctrl, the hemostasis time of LPRP-CP, Chito P and Chito G group was significantly shorter (P<0.001), and the amount of blood loss of LPRP-CP and Chito G group was decreased (P<0.05). Compared with LPRP-CP, there were no significant differences in hemostatic time and blood loss between Chito P and Chito G group (P>0.05). In the model of superficial Ⅱ° scalded on the back of rabbit, the wound healing rate of LPRP-CP was faster than that of the other three groups at the same time, and the healing effect was perfect. In the antibacterial test in vitro, only LPRP-CP had better anti-S. aureus effect, and all groups had no anti-E. coli effect.
CONCLUSION:LPRP-CP is an excellent hemostatic material for superficial wounds, and has certain antibacterial and wound healing effects, which has a wide academic value and research prospects.
