Identification of Weak D Type 1 in Rh Blood Group System and Discussion of Transfusion Strategy.
10.19746/j.cnki.issn.1009-2137.2022.03.032
- Author:
Ruo-Yang ZHANG
1
;
Jian-Yu XIAO
1
;
Qiang FENG
2
;
Cheng-Yin HUANG
1
;
Wen-Yi DING
1
;
Qing CHEN
3
Author Information
1. Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
2. Department of Clinical Laboratory, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, China.
3. Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China ；Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu Province, China,E-mail: chenqing90@yahoo.com.
- Publication Type:Journal Article
- Keywords:
RHD*01W.01;
Rh blood group;
genotyping
- MeSH:
Alleles;
Blood Transfusion;
Exons;
Genotype;
Humans;
Phenotype;
Rh-Hr Blood-Group System/genetics*
- From:
Journal of Experimental Hematology
2022;30(3):861-864
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the molecular mechanism of one patient with abnormal serological phenotype in RhD and discuss the transfusion strategy.
METHODS:The RhD variant sample was screened from a patient with IgM type anti-D antibody and further determined by three different sources of anti-D antibodies. Ten exons and the adjacent introns of the RHD gene were amplified, purified and sequenced. RhCE phenotypes and RHCE genotypes were detected.
RESULTS:The patient with Rh variant showed abnormal results of serological tests. The RHD gene sequence analysis showed that the RHD*01W.01 with a variation (c.809T>G, p.Val270Gly) in exon 6 of the RHD gene was found in the patient. The RhCE phenotype was CcEe. The genotyping results of RHCE were consistent with the serological typing results.
CONCLUSION:The Rh variant of the patient is RHD*01W.01, these findings indicate that RhD variants should be analyzed by molecular assays for the sake of safe transfusion.
