Effect of γδ T cells on the Proliferation, Apoptosis and Autophagy of Multiple Myeloma Cells.
10.19746/j.cnki.issn.1009-2137.2022.03.022
- Author:
Lin-Fen TAO
1
;
Bing-Qin YANG
2
;
Zhi-Yong ZENG
2
;
Jian-Ping XU
1
;
Dong-Hong LIN
1
;
Qiu-Chun CHEN
3
;
Jun-Min CHEN
4
Author Information
1. Department of Laboratory Medicine, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004， Fujian Province， China.
2. Department of Hematology， The First Affiliated Hospital of Fujian Medical University， Fuzhou 350005， Fujian Province， China.
3. Department of Anesthesiology， Union Hospital of Fujian Medical University， Fuzhou 350001， Fujian Province， China.
4. Department of Hematology， The First Affiliated Hospital of Fujian Medical University， Fuzhou 350005， Fujian Province， China,E-mail:drjunminchen@hotmail.com.
- Publication Type:Journal Article
- Keywords:
apoptosis;
autophagy;
myeloma cells;
proliferation;
γδ T cells
- MeSH:
AMP-Activated Protein Kinases/pharmacology*;
Apoptosis;
Autophagy;
Beclin-1/pharmacology*;
Cell Proliferation;
Humans;
Leukocytes, Mononuclear/metabolism*;
Multiple Myeloma/metabolism*;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
T-Lymphocytes;
TOR Serine-Threonine Kinases/metabolism*
- From:
Journal of Experimental Hematology
2022;30(3):797-803
- CountryChina
- Language:Chinese
-
Abstract:
AbstractObjective: To investigate the effect of γδ T cells on the proliferation, apoptosis and autophagy of multiple myeloma cells.
METHODS:Peripheral blood mononuclear cells (PBMNC) were isolated from healthy volunteers, and stimulated with zoledronic acid (Zol) in combination with rhIL-2. Flow cytometry analysis was used to detected the purity of γδ T cells. γδ T cells were collected and co-cultured with RPMI-8226 or U-266 cells at different effector target ratios. The proliferation of RPMI-8226 or U-266 cell lines were detected by CCK-8. Cell cycle and cell apoptosis were detected by flow cytometry and Western blot．The expressions of autophagy-related proteins were detected by Western blot.
RESULTS:γδ T cells can be expanded in vitro. γδ T cells could inhibit the proliferation of RPMI-8226 or U-266 cells, induced cell cycle arrest and promoted apoptosis in an effector target-dependent manner. In addition, γδ T cells could induce autophagy of myeloma cells, inhibited the expression of autophagy-related PI3K, P-AKT and P-mTOR, while increased the expression of AMPK and Beclin-1.
CONCLUSION:γδ T cells can inhibit the proliferation of RPMI-8226 and U-266 myeloma cells, induce cell cycle arrest, promote apoptosis, and enhance autophagy in vitro. The mechanism may be related to inhibition of PI3K/AKT/mTOR signaling pathway and/or activation of AMPK/Beclin-1 signaling pathway.
