Effect of polydatin on the proliferation and apoptosis of THP-1 cells and the mechanism.
10.7499/j.issn.1008-8830.2202078
- Author:
Chun-Mei WANG
1
;
Wen-Jing QI
1
;
Yan-Jiao REN
1
;
Guang-Yao SHENG
1
Author Information
1. Children's Hospital, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell proliferation;
Leukemia;
Polydatin;
THP-1 cell
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Glucosides/pharmacology*;
Humans;
Phosphatidylinositol 3-Kinases;
Proto-Oncogene Proteins c-akt;
Signal Transduction;
Stilbenes/pharmacology*;
THP-1 Cells;
TOR Serine-Threonine Kinases
- From:
Chinese Journal of Contemporary Pediatrics
2022;24(7):821-825
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To explore the effect of polydatin on the proliferation and apoptosis of acute monocytic leukemia cell line THP-1 and the possible mechanism.
METHODS:After THP-1 cells were treated with polydatin at gradient concentrations for 24 hours and 48 hours, their proliferation was determined by CCK-8 assay, and half maximal inhibitory concentration (IC50) was calculated. Logarithmically growing THP-1 cells were divided into two groups, a polydatin treatment group (treated with IC50 of polydatin) and a blank control group (treated without polydatin solution), and incubated for 48 hours. Cell apoptosis and cell cycle were measured by flow cytometry. The expression levels of PI3K, AKT, p-AKT, mTOR, p-mTOR, p70 S6K, and p-p70 S6K proteins were measured by Western blotting.
RESULTS:After treatment with polydatin, the proliferation of THP-1 cells was strongly inhibited, and the IC50 at 48 hours was 1 800 μmol/L. After treatment with 1 800 μmol/L polydatin solution for 48 hours, the apoptosis rate of THP-1 cells increased significantly compared with the blank control group (P<0.05). The cell cycle was arrested in the G0/G1 and S phases, with a significantly increased proportion of cells in the G0/G1 phase and a significantly decreased proportion of cells in the S phase, as compared with the blank control group (P<0.05). The expression levels of PI3K, AKT, p-AKT, mTOR, p-mTOR, p70 S6K, and p-p70 S6K proteins decreased significantly compared with the blank control group (P<0.05).
CONCLUSIONS:Polydatin can effectively inhibit the proliferation, block the cell cycle, and induce the apoptosis of THP-1 cells, which may be related to inhibition of the PI3K/AKT/mTOR signaling pathway.
