Biocompatibility evaluation of electrospun PLCL/fibrinogen nanofibers in anterior cruciate ligament reconstruction.
10.7507/1001-5515.202107011
- Author:
Jiahua GUO
1
;
Yu ZHANG
2
;
Liyuan CHEN
2
;
Liming XU
2
;
Xiumei MO
1
;
Liang CHEN
2
Author Information
1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China.
2. National Institutes for Food and Drug Control, Beijing 102629, P. R. China.
- Publication Type:Journal Article
- Keywords:
Biocompatibility;
Electrospun;
Ligament reconstruction;
PLCL/Fibrinogen nanofibers
- MeSH:
Anterior Cruciate Ligament Reconstruction;
Cell Adhesion;
Fibrinogen;
Materials Testing;
Nanofibers
- From:
Journal of Biomedical Engineering
2022;39(3):544-550
- CountryChina
- Language:Chinese
-
Abstract:
The study aimed to evaluate the safety and function of poly(lactic-acid-co-ε-caprolactone) (PLCL)/fibrinogen nanofibers (P/F-Ns), and provide theoretical basis for the clinical application. The surface morphology, mechanical properties, the hydrophilicity and the fibrinogen content of P/F-Ns were tested by scanning electron microscope, the material testing machine, the contact angle meter and the microplate reader, respectively. The cell adhesion, proliferation and ligament remodeling genes expression of Hig-82 cells on P/F-Ns were conducted through cell counting kit-8 (CCK-8) and real-time quantitative PCR analyses, respectively. The results showed that with the increase of the fibrinogen content, the pore sizes and hydrophilicity of three P/F-Ns increased, but the mechanical properties decreased. Cell adhesion and proliferation tests showed that P/F-N-2 held the best ability to promote cell adhesion and proliferation. The ligament remodeling genes expressions of Hig-82 cells on P/F-N-1, P/F-N-2 and P/F-N-3 were all up-regulated compared to P/F-N-0 on days 3 and 7. All the three P/F-Ns containing fibrinogen (P/F-N-1, P/F-N-2 and P/F-N-3) had better biocompatibility compared to P/F-N-0, and could be efficiently applied to the reconstruction of anterior cruciate ligament.
