Anti-inflammation effect of moxibustion for rats with diarrhea-predominant irritable bowel syndrome based on multiple miRNAs regulating NF-κB signal pathway.
10.13703/j.0255-2930.20210521-k0002
- Author:
Shan-Shan ZHU
1
;
Jiao-Jiao WANG
1
;
Ling ZOU
1
;
Jin-Yu CHEN
1
;
Kui-Wu LI
1
;
Lu-Min LIAO
1
;
Jing-Ru RUAN
1
;
Nan LI
2
,
3
;
Hao-Ran CHU
2
,
4
,
5
Author Information
1. Graduate School of Anhui University of CM, Hefei 230038, China.
2. Second Affiliated Hospital of Anhui University of CM, Hefei 230061
3. Anhui Acupuncture Clinical Medical Research Center, Hefei 230061.
4. Anhui Acupuncture Clinical Medical Research Center, Hefei 230061
5. Clinical Institute of Acupuncture and Moxibustion, Anhui Academy of TCM, Hefei 230038.
- Publication Type:Journal Article
- Keywords:
diarrhea-predominant irritable bowel syndrome;
microRNAs (miRNAs);
moxibustion;
nuclear factor-kappa B (BNF-κB)
- MeSH:
Animals;
Diarrhea/therapy*;
Interleukin-8/genetics*;
Irritable Bowel Syndrome/therapy*;
MicroRNAs/genetics*;
Moxibustion;
NF-kappa B/metabolism*;
RNA, Messenger;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Tumor Necrosis Factor-alpha/genetics*
- From:
Chinese Acupuncture & Moxibustion
2022;42(6):654-662
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of moxibustion on the regulation of nuclear factor-kappa B (NF-κB) and inflammatory factors by multiple microRNAs (miRNAs) in rats with diarrhea-predominant irritable bowel syndrome (IBS-D), and to explore the anti-inflammatory mechanism of moxibustion on IBS-D.
METHODS:Twelve of 52 newborn rats were randomly selected into a normal group. The remaining rats were made into IBS-D model. A total of 36 rats with successful model were randomly divided into a model group, a medication group and a moxibustion group, 12 rats in each group. The rats in the medication group were intraperitoneally injected with pyrrolidine dithiocarbamate (PDTC). The rats in the moxibustion group were treated with moxibustion at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) for 20 min each time. All the intervention was given once a day for 7 days. Before and after modeling as well as after intervention, the body mass, loose stool rate and the minimum volume threshold of abdominal withdrawal reflex (AWR) were measured. After intervention, the contents of serum tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-8 were detected by ELISA method; the morphology of colon tissues was observed by HE staining, and the expressions of miR-155, miR-125b, miR-29b, miR-31, miR-18a and NF-κB p65 mRNA in colon tissues were detected by real-time PCR. The expressions of NF-κB p65, TNF-α, IL-1β and IL-8 protein in colon tissues were detected by immunofluorescence.
RESULTS:After modeling, the body mass and the minimum volume threshold of AWR in the model group were lower than those in the normal group (P<0.01); the rates of loose stool in the model group were higher than those in the normal group (P<0.01); after intervention, in the model group, the inflammatory infiltration of colon tissues was obvious, and the serum levels of TNF-α, IL-1 β, IL-8 were higher than those in the normal group (P<0.05); the expression of miR-155, miR-125b, miR-29b, miR-31, miR-18a and NF-κB p65 mRNA in colon tissues was higher than that in the normal group (P<0.05); the protein expression of NF-κB p65, TNF-α, IL-1β, IL-8 was also higher than that in the normal group (P<0.01). After intervention, the body mass and the minimum volume threshold of AWR in the medication group and the moxibustion group were both higher than those in the model group (P<0.05); the loose stool rate in the medication group and the moxibustion group were lower than those in model group (P<0.05); the inflammatory cells infiltration in the colon tissues was less, the serum levels of TNF-α, IL-1β and IL-8 as well as the protein expression of NF-κB p65, TNF-α, IL-1β and IL-8 in the colon tissues in the medication group and the moxibustion group were lower than those in the model group (P<0.05, P<0.01). The expression of miR-125b, miR-31, miR-18a and NF-κB p65 mRNA in the medication group were lower than those in the model group (P<0.05). The expression of miR-155, miR-125b, miR-29b, miR-31, miR-18a and NF-κB p65 mRNA in the moxibustion group were lower than those in the model group (P<0.05). The miR-155, miR-125b, miR-29b, miR-31, miR-18a were positively correlated with NF-κB p65 mRNA (0<r<1, P<0.01).
CONCLUSION:The anti-inflammatory mechanism of moxibustion at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) for IBS-D rats may be related to regulating multiple miRNAs to inhibit NF-κB signal pathway and reduce the expression of inflammatory factors.
