The Risk of Gastrointestinal Cancer on Daily Intake of Low-Dose BaP in C57BL/6 for 60 Days
10.3346/jkms.2022.37.e235
- Author:
Zhi ZHENG
1
;
Jung Kuk PARK
;
Oh Wook KWON
;
Sung Hoon AHN
;
Young Joo KWON
;
Linjuan JIANG
;
Shaohui ZHU
;
Byoung Hee PARK
Author Information
1. School of Public Health, Xinxiang Medical University, Henan, China
- Publication Type:Original Article
- From:Journal of Korean Medical Science
2022;37(30):e235-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Benzo(a)pyrene (BaP) is a carcinogenic compound in contaminated foodstuffs.The effect of oral intake of the environmental carcinogen BaP under low doses and frequent exposure on a digestive system has not been thoroughly verified.
Methods:In this regard, this study was conducted to prove the toxicity effects of BaP on the stomach and colon tissue after exposure to C57BL/6 mouse (3 and 6 µg/kg) following daily oral administration for 60 days. This study investigated acute gastric mucosal injury, severe gastric edema, cell infiltration, and mononuclear cells, multifocal cells, and tumoral inflammatory cells.
Results:The results of ELISA showed that the expression of serum interleukin (IL)-6 and tumor necrosis factor-α in the BaP exposure group were significantly increased, and a high level of DNA adduct distribution in their stomach and colon. Moreover, this study has confirmed the expression of early carcinogenesis markers: nuclear factor (NF)-κB, p53, IL-6, superoxide dismutase 1 (SOD1), mucin (MUC1 and MUC2), and β-catenin in the stomach and colon, and showed that there was a significant increase in IL-6, NF-κB, SOD1, β-catenin, and MUC1 (P< 0.05). At the same time, there was a significant decrease in MUC2 and p53 (P < 0.05). Thus, even in low doses, oral intake of BaP can induce DNA damage, increasing the potential risk of gastrointestinal cancer.
Conclusion:This study will provide a scientific basis for researching environmental contaminated food and intestinal health following daily oral administration of BaP.
