Identification of Stem Cell Related Gene Expression from the Osteosarcoma Cell Core Side
10.15430/JCP.2022.27.2.122
- Author:
Chaemoon LIM
1
;
Young Ho ROH
;
Seung Jin YOO
;
Dong Kee JEONG
;
Kwang Woo NAM
Author Information
1. Department of Orthopaedic Surgery, Jeju National University Hospital, Jeju, Korea
- Publication Type:Original Article
- From:Journal of Cancer Prevention
2022;27(2):122-128
- CountryRepublic of Korea
- Language:English
-
Abstract:
Osteosarcoma is the most frequent primary malignant bone tumor with higher incidences in children and adolescents. Despite clinical evolutions, patients with osteosacoma have had a poor prognosis. There has been increasing evidence that cancer is a stem cell disease. This study sought to isolate and characterize cancer stem cells from human osteosarcoma with relevant literature reviews. Here we show that the emerging evidence suggests osteosarcoma should be regarded as a differentiation disease such as stem cell disease. Two human osteosarcoma cell lines were cultured in non-adherent culture conditions as sarcospheres. Sarcospheres were observed using histomorphology and alkaline phosphatase (ALP) staining. Expression of the embryonic stem cell marker was analyzed with use of reverse transcriptase-PCR. Sarcospheres could be reproduced consistently throughout multiple passages and produced adherent osteosarcoma cell cultures. Expression of stem cell-associated genes such as those encoding Nanog, octamer-binding transcription factor 3/4, sex determining region Y box 2 , c-Myc and ALP indicated pluripotent stem-like cells. These results support the extension of the cancer stem cell theory to include osteosarcoma. Understanding the cancer stem cell derived from human osteosarcoma could lead to the evolution of diagnosis and treatment for osteosarcoma patients.
