The Effect of alpha-Melanocyte Stimulating Hormone on Renal Ischemia Reperfusion Injury.
- Author:
Cha Kyong YOM
1
;
Jun Woo JIN
;
Ku Yong CHUNG
;
Sun Hee SUNG
;
Ki Whan HAN
;
Eun Suk KANG
;
Yu Seun KIM
Author Information
1. Department of Surgery, Ewha Womans University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Ischemia-reperfusion injury;
Acute renal failure;
alpha-MSH
- MeSH:
Acute Kidney Injury;
alpha-MSH;
Animals;
Chemokines;
Creatinine;
Cytokines;
Humans;
Ischemia*;
Male;
Mortality;
Neutrophil Infiltration;
Nitric Oxide;
Rats;
Reperfusion;
Reperfusion Injury*;
Survival Rate
- From:The Journal of the Korean Society for Transplantation
2006;20(1):49-54
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In ischemia-reperfusion induced renal injuries, cytokines, chemoattractant chemokines, adhesion molecules and nitric oxide play an important role. alpha-Melanocyte stimulating hormone (alpha-MSH) is a potent anti-inflammatory cytokine so the therapeutic effect of alpha-MSH on an ischemia-reperfusion induced acute renal failure is to be evaluated. METHODS: 40 male Spraque-Dawley rats were prepared for the experiment, they were classified into three classes (Sham, ischemic control and alpha-MSH injection). Both renal pedicles were clamped for 45 minutes. alpha-MSH (50 microgram) was injected intravenously three times, immediately before ischemia and reperfusion and 18 hour after reperfusion. Serum creatinine and histologic changes were analyzed between groups (Sham (n=6), ischemic control group (n=15), and alpha-MSH group (n=19)). RESULTS: Serum creatinine level decreased significantly at 24 hours after reperfusion in alpha-MSH treated animals (SCr24 0.78+/-0.23 mg/dL, 4.21+/-1.14 mg/dL, 3.01+/-1.19 mg/dL, repectively (P=0.008)), especially serum creatinine level at 48 hours after reperfusion much more dicreased in alpha-MSH group (SCr48 0.67+/-0.16 mg/dL, 4.21+/-2.03 mg/dL, 1.15+/-1.11 mg/dL, repectively (P=0.004)). Tubular neccrosis and neutrophil infiltration decreased signigicantly in alpha-MSH treated group (P=0.001). Mortality was noted 33.3% only at ischemic conrol group. CONCLUSION: we demonstrate the fact that alpha-MSH has protective role on ischemic renal injury and improves survival rates.
