Molecular interactions between the main components of Shuanghuanglian injection and ciprofloxacin injection based on self-assembly
10.16438/j.0513-4870.2022-0696
- VernacularTitle:基于自组装体系研究双黄连主要成分与环丙沙星的分子互作
- Author:
Jiang-ling LI
1
;
Shuang LIU
2
,
3
;
Yi-qing XIE
4
;
Jiang-lan LONG
2
,
3
;
Zhen-zhen WANG
2
,
3
;
Ai-ting WANG
2
,
3
;
Qiang MA
4
;
Dan YAN
2
,
3
Author Information
1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
3. Beijing Institute of Clinical Pharmacy, Beijing 100050, China
4. Chinese Academy of Inspection and Quarantine, Beijing 100176, China
- Publication Type:Research Article
- Keywords:
Shuanghuanglian injection;
ciprofloxacin injection;
self-assembly;
molecular interaction;
antibacterial effect
- From:
Acta Pharmaceutica Sinica
2022;57(8):2445-2452
- CountryChina
- Language:Chinese
-
Abstract:
The combination of Shuanghuanglian injection (SHLI) and ciprofloxacin injection (CIPI) is frequently prescribed in clinical practice, but the basis for the combination is weak. In this study, isothermal titration calorimetry and ultraviolet-visible absorption spectrometry were applied to identify the molecular interactions of SHLI and its main components, chlorogenic acid and neochlorogenic acid with CIPI. Scanning electron microscopy, Fourier-transform infrared spectroscopy, and cold-spray ionization mass spectrometry were performed to confirm that this molecular interaction was related to the formation of self-assembled supramolecular systems induced by chlorogenic acid and neochlorogenic acid with CIPI through weak intermolecular bonds. The antibacterial activity toward Pseudomonas aeruginosa (P. aeruginosa) was evaluated via molecular interactions, and the inhibitory ability of SHLI, chlorogenic acid and neochlorogenic acid against P. aeruginosa was significantly reduced after interaction with CIPI. A molecular docking study demonstrated that the reduced antibacterial ability was closely related to the competitive binding of drug molecules to the same binding site of the DNA gyrase B (GyrB) subunit of P. aeruginosa. The present study uncovered the intermolecular interactions of SHLI and its main components chlorogenic acid and neochlorogenic acid with CIPI from the perspective of molecular self-assembly and contribute to the reduction of its antibacterial ability, providing a basis for the clinical combination of SHLI and CIPI.
