SNP double peak methods through chloroplast genome profiling for identifying Gentiana crassicaulis and two closely related species
10.16438/j.0513-4870.2022-0496
- VernacularTitle:基于叶绿体全基因组序列的双峰法鉴定粗茎秦艽及其近缘物种
- Author:
Na-na GAO
1
;
Liang-hong NI
1
;
Zhi-li ZHAO
1
;
Dorje GAAWE
2
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Tibetan Traditional Medical College, Lhasa 850000, China
- Publication Type:Research Article
- Keywords:
italic>Gentiana tibetica;
italic>Gentiana crassicaulis;
italic>Gentiana robusta;
chloroplast genome;
SNP marker;
ouble peak detection
- From:
Acta Pharmaceutica Sinica
2022;57(8):2520-2527
- CountryChina
- Language:Chinese
-
Abstract:
italic>Gentiana crassicaulis Duthie ex Burk. is one of the plant sources of Gentianae Macrophyllae Radix (QinJiao). Gentiana tibetica King ex Hook. f. and Gentiana robusta King ex Hook. f. are relative species of G. crassicaulis. Due to the large intraspecific morphological variation, G. crassicaulis showed high morphological similarity with G. tibetica and G. robusta. And the distribution area of the three species overlaps to some extent, which makes it difficult to identify them. On the basis of morphological identification, the method of molecular identification of the three species was constructed in this study based on chloroplast genomes. The chloroplast genome of Gentiana tibetica is 148 765bp long, with LSC, SSC and IR 81 163 bp, 17 070 bp and 25 266 bp, respectively. The structure of the three is consistent. The chloroplast genome sequences of G. tibetica and G. crassicaulis are highly similar, and the number of variable sites is 9 (149 267 bp in total). Diagnostic SNP that could effectively identify the three species was screened and verified, and a dual-peak SNP detection method was established for the effective identification of each species and mixed samples. Our study provides basic data for the molecular identification of G. crassicaulis and its related species, and the arrangement of related Tibetan medicine.
