The identification of <italic>bZIP</italic> gene family in <italic>Cannabis sativa</italic> L. and its preliminary research of the function in regulation of lipid metabolism

Hao Huai; Kang Ning; Cong Hou; Shu-ming Yang; Jun-zhi Wang; Shi-lin Chen; Lin-lin Dong

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The identification of bZIP gene family in Cannabis sativa L. and its preliminary research of the function in regulation of lipid metabolism

VernacularTitle:火麻仁基原植物bZIP基因家族鉴定及其调控油脂代谢的功能初探
Author: Hao HUAI ^{1
,

2
,

3} ; Kang NING ⁴ ; Cong HOU ⁴ ; Shu-ming YANG ⁵ ; Jun-zhi WANG ^{1
,

2} ; Shi-lin CHEN ⁴ ; Lin-lin DONG ⁴
Author Information

1. College of Biological &
2. Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China
3. Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
4. Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
5. Yunnan Hemp Industrial Investment Co. Ltd., Kunming 650217, China
Publication Type:Research Article
Keywords: hemp seed; non-psychoactive medicinal cannabis; italic>bZIP gene family; lipid metabolism; expression profile
From: Acta Pharmaceutica Sinica 2022;57(8):2528-2542
CountryChina
Language:Chinese
Abstract: The bZIP (basic leucine zipper) gene family is one of the largest transcription factor families in eukaryotes, and its members play important roles in stress response, secondary metabolism, plant growth, seed development and other aspects. To investigate the biological functions of the bZIP (CsbZIP) gene in Cannabis sativa L., we systematically investigated the CsbZIP gene family using bioinformatics methods based on the whole-genome and transcriptome data. The results showed that 55 CsbZIP gene family members (CsbZIP1-CsbZIP55) were identified and distributed on 10 chromosomes, belonging to 12 subfamilies. The gene structure and protein motif distribution of the same subfamily members were similar. Segment repeats were the main reasons for the expansion of CsbZIP gene family. Cis-elements analysis showed that the promoter regions of 73 lipid synthesis genes contained G-box or A-box element. qRT-PCR showed that the relative expression levels of 7 CsbZIP genes and 7 lipid synthesis genes were relatively high in hemp seed. 7 CsbZIP genes had a significant positive correlation with 7 lipid synthesis genes. This study revealed the structural features, evolutionary patterns and expression patterns of CsbZIP, providing important clues for further study on the regulation of CsbZIP on oil metabolism of hemp seed.