Enzyme-responsive fluorescent probes for bacteria imaging
10.16438/j.0513-4870.2022-0303
- VernacularTitle:细菌代谢酶响应型荧光探针研究进展
- Author:
Rao WEI
;
Hai-yu HU
;
Lei-lei ZHANG
- Publication Type:Research Article
- Keywords:
enzyme;
bacteria;
fluorescent probe;
biological imaging;
probe design
- From:
Acta Pharmaceutica Sinica
2022;57(8):2313-2326
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescent probes are potential fluorophores that display signals based on the changes in tissue microenvironment, interactions with analytes or specific biochemical reactions. Metabolic enzymes are the most important protein involved in bacteria activities. Complex dynamics of biological processes in bacteria are elucidated by these metabolic enzymes-based fluorescent probes with high spatial resolution and sensitivity. Here, we review recent advances in metabolic enzyme-responsive fluorescent probes for bacteria imaging. It was organized according to enzyme classification systems, focused on fluorescence masking strategies, molecular mechanisms of enzyme activation, and bio-related applications.
