Effects of irbesartan combined with 5-fluorouracil on the proliferation and ERK/PPARγ signaling pathway of Lewis lung cancer cells
- VernacularTitle:厄贝沙坦联合5-氟尿嘧啶对Lewis肺癌细胞增殖及ERK/PPARγ信号通路的影响
- Author:
Meijuan ZHAI
1
;
Shiliang JI
2
;
Yiguo JIANG
2
;
Xiuhua BAI
2
Author Information
1. Dept. of Pharmacy，the Affiliated Suzhou Hospital of Nanjing Medical University/Suzhou Municipal Hospital，Jiangsu Suzhou 215002，China
2. Dept. of Respiratory Medicine，Suzhou Scie nce & Technology Town Hospital，Jiangsu Suzhou 215000，China
- Publication Type:Journal Article
- Keywords:
irbesartan;
5-fluorouracil;
lung cancer cell
- From:
China Pharmacy
2022;33(16):1986-1989
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effects of irbesartan（Irb）combined with 5-fluorouracil（5-FU）on the proliferation and extracellular signal-regulated kinase （ERK）/peroxidase proliferator-activated receptor γ（PPARγ）signaling pathway of Lewis lung cancer cells. METHODS Lewis lung cancer cells from mice were divided into normal control （NC）group，Irb low-dose （LD）group（1×10－3 mmol/L），Irb high-dose （HD）group（1×10－1 mmol/L），5-FU group （10 μmol/L），Irb LD+ 5-FU group （Irb 1×10－3 mmol/L+5-FU 10 μmol/L）and Irb HD+ 5-FU group （Irb 1×10－1 mmol/L+5-FU 10 μmol/L）. MTT method was used to measure the activity of cell proliferation in each group. Plate colony formation experiment was used to determine the number of cell colonies formed in each group ；Western blot method was used to detect the expression levels of proliferating cell nuclear antigen （PCNA），p53，ERK1/2，p-ERK1/2 and PPAR γ protein in each group. RESULTS Compared with the NC group ，the cell proliferation activity ，the number of colonies formed and the protein levels of PCNA ，p-ERK1/2，and PPARγ were significantly reduced in the other five groups ，and the protein level of p 53 was significantly increased （P＜0.05）；the protein expression of ERK1/2 had no significant difference （P＞0.05）. The changes of above indexes in Irb LD+ 5-FU group and Irb HD+ 5-FU group were more significant than Irb LD group ，Irb HD group and 5-FU group （P＜0.05）. CONCLUSIONS Irb combined with 5-FU can inhibit the proliferation of Lewis lung cancer cell ，and the effect is better than that of the two alone. The mechanism may be related to the inhibition of ERK/PPARγ signal pathway.
