Establishment of characteristic chromatogram and content determination of 4 index components in Jianpi yifei biyan prescription standard decoction
- VernacularTitle:健脾益肺鼻炎方标准汤剂特征图谱的建立及4种指标成分的含量测定
- Author:
Xin WAN
1
;
Detang LI
2
;
Lijuan ZHANG
3
;
Meirong YI
2
;
He ZHU
2
;
Jialun HE
2
;
Jie CHEN
2
;
Hongmei TANG
2
;
Zhenwen QIU
2
Author Information
1. The First Clinical Medical College，Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. Dept. of Pharmacy，the First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510405，China
3. Dept. of Otolaryngology，the First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510405，China
- Publication Type:Journal Article
- Keywords:
Jianpi yifei biyan prescriptio n;
standard decoction;
characteristic chromatogram;
stoichiometric analysis
- From:
China Pharmacy
2022;33(16):1980-1985
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish HPLC characteristic chro matogram of Jianpi yifei biyan prescription standard decoction ， to select the quality control index components and determine their contents. METHODS HPLC method combined with Similarity Evaluation System of TCM Chromatographic Fingerprint （2004 edition）were used to establish the characteristic chromatogram of 10 batches of Jianpi yifei biyan prescription standard decoction ；the similarity evaluation and common peaks identification were also carried out. Using common peak area of characteristic chromatogram as variables ，SPSS 26.0 software and SIMCA 14.1 software were used to perfor m cluster analysis （CA），principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA）；differential components with variable important i n pro jection（VIP）value greater than 1.5 were screened；the contents of cimifugin and differential components were determined by the same method. RESULTS A total of 24 common characteristic peaks were identified ， and the similarities of 10 batches of samples were higher than 0.960；eight characteristic peaks were identified by comparison with reference substance. CA and PCA results revealed that the samples were classified into 3 categories.OPLS-DA analysis showed that 3 components with VIP value greater than 1.5， which were prim-O-glucosylcimifugin （peak 2），calycosin 7-O-β-D-glucopyranoside （peak 4） and 5-O-methylvisammioside （peak 6） in descending order. The linear ranges of prim- O- glucosylcimifugin，calycosin 7-O-β-D-glucopyranoside，cimifugin and 5-O-methylvisammioside were 0.010 7-0.213 0，0.007 8- 0.156 0，0.008 0-0.160 0，0.009 8-0.195 0 μg（r＞0.999），respectively. RSD values of precision ，repeatability and stability tests （24 h） were all less than 2％. Average recoveries were 105.98％（RSD＝1.75％，n＝6），98.06％（RSD＝3.87％，n＝6），96.38％（RSD＝ 4.03％ ，n＝6） and 104.17％（RSD＝1.27％ ，n＝6）. The contents of the above 4 components in 10 batches of samples were 12.12-18.87，3.86-6.40，3.10-4.27 and 11.17-15.79 μ g/mL，respectively. CONCLUSIONS The established HPLC characteristic chromatographic method is stable and feasible ，it can be used for the quality control of Jianpi yifei biyan prescription standard decoction. Prim- O-glucosylcimifugin，calycosin 7-O-β-D-glucopyranoside，cimifugin and 5-O-methylvisammioside can be used as the index components for quality control of the standard decoction.
