Content determination of 6 components in Melastoma dodecandrum and analysis of its correlation with antioxidant activity
- VernacularTitle:地稔中6种成分含量测定及其与抗氧化活性的相关性分析
- Author:
Jing LIU
1
;
Huiyi MING
2
;
Xiuping MA
1
,
3
,
4
;
Song QIAN
1
;
Jing YANG
1
,
4
;
Dengli WU
1
;
Jiangtao GUO
1
Author Information
1. School of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. Dept. of Pharmacy，Zhijin County People’s Hospital，Guizhou Bijie 552100，China
3. National Miao Medicine Engineering Technology Research Center/Guizhou Traditional Chinese Medicine Processing and Preparation Engineering Technology Research Center，Guiyang 550025，China
4. Tea + Big Health Food Development and Research Center，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Melastoma dodecandrum;
content detemination;
QAMS;
antioxidant activity;
correlation
- From:
China Pharmacy
2022;33(16):1962-1967
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for simultaneous determi nation of six components in Melastoma dodecandrum and investigate its correlation with antioxidant activity. METHODS Ultra high-performance liquid chromatography （UPLC）method was adopted. The contents of gallic acid ，protocatechuic acid ，isovitexin，rutin and ellagic acid in 23 batches of M. dodecandrum were determined by quantitative analysis of multi-components by single-marker （QAMS）method，using vitexin as the internal reference. Then 1，1-diphenyl-2-picrylhydrazyl（DPPH）free radical method ，2，2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid ） diammonium salt （ABTS）method and ferric ion reduction/antioxidant power （FRAP）method were applied to determine the antioxidant activity of 23 batches of M. dodecandrum . Grey correlation analysis and bivariate correlation analysis were used to evaluate the correlation between six components and antioxidant activity. RESULTS The content of vitexin were 0.021％-0.182％. The contents of gallic acid ，protocatechuic acid ，isovitexin，rutin and ellagic acid by QAMS method were 0.008％-0.042％， 0.003％-0.023％，0.071％-0.283％，0.013％-0.140％ and 0.006％-0.021％，respectively. Compared with the results of external standard method ，P was greater than 0.05. Grey correlation analysis showed that the grey correlation coefficients between the contents of six components an d antioxidant activity was 0.727 6- 0.866 9. Bivariate correlation analysis showed that the contents of vitexin ，isovitexin and rutin were positively correlated with antioxidant activity （P＜0.05 or P＜0.01），the content of gallic acid and protocatechuic acid were negatively correlated with antioxidant activity （P＜0.05）. There was no significant correlation between ellagic acid and antioxidant activity.CONCLUSIONS QAMS method is successfully established for the simultaneous determination of six components in M. dodecandrum. The six components in M. dodecandrum are highly correlated with antioxidant activity.
