The mechanism of effective fractions from Xiongma decoction in the treatment of migraine model rats with hyperactivity of liver-yang and blood stasis
- VernacularTitle:芎麻汤有效部位防治肝阳上亢兼瘀血型偏头痛的作用机制
- Author:
Dingding LIU
1
;
Dong LIU
1
,
2
;
Lulu CHANG
1
;
Jingru WANG
1
;
Xuemei WU
1
;
Jiansheng GUO
2
;
Guirong ZENG
3
Author Information
1. College of Pharmacy，Guizhou University of Traditional Chinese Medicine & Research Center for Pharmacodynamic Material Basis and Mechanism of Action，Guiyang 550025，China
2. College of Pharmacy，Hunan University of Chinese Medicine，Changsha 410208，China
3. Hunan Provincial Research Center for Safety Evaluation of Drugs & Hunan Key Laboratory of Pharmacodynamics and Safety Evaluation of New Drugs，Changsha 410331，China
- Publication Type:Journal Article
- Keywords:
Xiongma decoction;
effective fraction;
hyperactivity of liver-yang;
migraine of blood stasis;
transient receptor
- From:
China Pharmacy
2022;33(16):1950-1955
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To expl ore the mechanism of effe ctive fractions from Xiongma decoction in the treatment of migraine with hyperactivity of liver-yang and blood stasis. METHODS Totally 70 male SD rats were randomly divided into normal group ， model group ，positive control group （Flunarizine hydrochloride capsules 0.9 mg/kg），low-dose and high-dose groups of Xiongma decoction effective fractions （ethyl acetate extract 0.87，3.46 g/kg，n-butanol extract 1.80，7.20 g/kg）. Except for normal group ， rats in other groups were given aconite decoction （2 g/kg），once a day ，for 4 consecutive weeks to establish the hyperactivity model of liver-yang. On the 15th day of modeling ，all administration groups were given corresponding drugs intragastrically at the same time ，once a day ，for 2 consecutive weeks. On the 29th day of modeling ，rats trigeminal ganglion was stimulated to establish the migraine model with hyperactivity of liver-yang and blood stasis ，then the medication was maintained for another time according to the above method. The macroscopic signs and behavior of the rats were observed ；positive expression ，mRNA and protein expression of transient receptor potential vanilloid 1 （TRPV1），calcitonin generelated peptide （CGRP），calcitonin receptor-like receptor （CRLR） and receptor-associated membrane proteins 1 （RAMP1） in trigeminal cervical spinal complex （TCC） were detected by immunohistochemistry ，RT-qPCR and Western blot assay. RESULTS Rats in model group showed macrophysical signs and behaviora l manifestations related to migraine with hyperactivity of liver-yang and blood stasis. Thirty minutes after last administration ，the above conditions of rats in Xiongma decoction effective fraction groups were improved significantly. Compared with normal group ， positive expression，mRNA and protein expression of TRPV 1，CGRP， CRLR and RAMP 1 in TCC of rats in the model group were significantly increased （P＜0.05）. Compared with model zengguirong@hnse.org group， most of above indicators in Xiongma decoction effective fraction groups were significantly decreased （P＜0.05）. CONCLUSIONS The mechanism of Xiongma decoction in preventing and treating migraine with hyperactivity of liver-yang and blood stasis may be related to inhibit the activity of TRPV1-CGRP/CGRP receptor signaling pathway in TCC.
