Identification of chemical constituents in Shenfu injection and study on anti-inflammatory activities of its poly- acetylene compounds
- VernacularTitle:参附注射液化学成分鉴定及其炔醇类化合物的抗炎活性研究
- Author:
Qi ZHENG
1
,
2
;
Xiaoya WANG
1
,
2
;
Jie LIU
1
,
2
;
Cheng PENG
1
;
Liang XIONG
1
,
2
;
Yaning ZHU
3
;
Qinmei ZHOU
1
,
2
Author Information
1. State Key Laboratory of Southwestern Chinese Medicine Resources/School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
2. Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
3. China Resources Sanjiu （Ya’an） Pharmaceutical Co.，Ltd.，Sichuan Ya’an 625000，China
- Publication Type:Journal Article
- Keywords:
Shenfu injection;
polyacetylenes;
anti-
- From:
China Pharmacy
2022;33(16):1931-1936
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the chemical constituents in S henfu injection and the anti-inflammatory activities of its polyacetylene compounds. METHODS Shenfu injection was separated and purified by macroporous adsorption resin ，medium pressure liquid chromatography ，preparative thin layer chromatography and reversed-phase semi-preparative high-performance liquid chromatography，and the compound structure was identified according to the physicochemical properties and spectral data. RAW 264.7 cell inflammation model was used to evaluate the anti-inflammatory activities of polyacetylene compounds . The effects of active polyacetylene compounds on the expressions of cyclooxygenase- 2（COX-2）protein were evaluated by Western blot assay. RESULTS Twelves compounds were isolated and identified from Shenfu injection ，including 8 ginsenoside compounds ，i.e. ginsenoside Rg 1（1），ginsenoside Re （2），ginsenoside Rb 1（3），ginsenoside Rk 1（4），20（R）-ginsenoside Rh 1（5），20（S）-ginsenoside Rg3 （6），notoginsenoside R 1（7），panaxatriol（8）；4 polyacetylene compounds ，i.e.（3R，9R，10R）-panaxytriol（9），panaxydol（10）， heptadeca-1，8-dien-4，6-diyne-3，10-diol（11）and panaxynol （12）. Among 4 polyacetylene compounds ，only compound 10 had anti-inflammatory activity. Compound 10 was not toxic to normal RAW 264.7 cells；when the concentration of compound 10 ranged 12.5-50.0 μmol/L，it could significantly reverse the lipopolysaccharide-induced NO content increase in cell supernatant （P＜0.05 or P＜0.01）；when the concentration of co mpound 10 was 50.0 μmol/L，it could significantly reverse the lipopolysaccharide-induced protein expression increase of COX- 2 in cells （P＜0.05）. CONCLUSIONS Compounds 4，7，10-12 are identified and reported in Shenfu injection for the first time ，and panaxydol possesses a certain anti-inflammatory effect.
