Effects of Heat Preconditioning on Expression of Protein Kinase C in Myocardium of Rats after Overload Exercise
10.3969/j.issn.1006-9771.2015.01.013
- VernacularTitle:热预处理对超负荷训练大鼠心肌蛋白激酶C表达的影响
- Author:
Yi YANG
;
Zhanghua LI
;
Wudi SHI
;
Hua LIU
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
heat preconditioning, myocardium, protein kinase C, overload exercise, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of heat preconditioning (HP) on the expression of protein kinase C (PKC) δ and PKCε in the myocardium of overload exercise rats. Methods 25 male three-month-old Sprague-Dauley rats were divided into control group (n=5), exercise group (n=10) and HP group (n=10). The expression of PKCδ and PKCε in the myocardium was detected with immunohistochemistry, real-time PCR and Western blotting 8 weeks after overload exercise. Results The expression of PKCδ significantly increased in the exercise group compared with the control group and the HP group (P<0.05). In another hand, PCR and immunohistochemistry showed the expression of PKCε in the exercise group decreased compared with the control group and the HP group (P<0.05), but it was not significantly different among the groups using Western blotting (P>0.05). Conclusion PKCs may play an important role in the HP-induced cardio-protection during overload exercise.
