Spatial Organization of Neurovascular Unit after Focal Cerebral Ischemia-reperfusion in Rats
10.3969/j.issn.1006-9771.2015.01.008
- VernacularTitle:局灶性脑缺血再灌注大鼠神经血管单元的空间构筑
- Author:
Yuanyuan JIN
;
Jing HU
;
Lu FENG
;
Yang LI
;
Yeyun CHEN
;
Xiaosong HU
;
Shuai LI
- Publication Type:Journal Article
- Keywords:
focal cerebral ischemia, reperfusion, neurovascular unit, spatial organization, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the spatial organization of neurons, blood vessel and astrocytes at different time of reperfusion after focal cerebral ischemia in rats. Methods 24 Sprague-Dawley rats were randomly divided into sham group (n=8), reperfusion 1 day group and reperfusion 2 weeks group. The latter 2 groups were occluded the middle cerebral arteries for an hour and reperfused. All the rats were injected with gelatin ink. The expressions of glial fibrillary acid protein (GFAP) and neuronal nuclear antigen (NeuN) in the brain were observed with immunohistochemistry. Results The vessels located mainly in cortex and nucleus. Most of astrocytes apophysis connected with vessels and neurons. Compared to the sham group, the expression of GFAP increased significantly in ischemic side, and the expression of NeuN decreased 1 day and 2 weeks after ischemia-reperfusion. The vessels decreased in the ischemic side 1 day after cerebral ischemia-reperfusion, and then increased 2 weeks later. Conclusion The organization of neurovascular unit after cerebral ischemia-reperfusion has been observed.
