Resibufogenin suppresses human hepatocellular carcinoma cell proliferation, migration and invasion in vitro
10.16438/j.0513-4870.2022-0119
- VernacularTitle:酯蟾毒配基抑制人肝癌细胞体外增殖和迁移侵袭研究
- Author:
Tong-shan QIAO
1
;
Wen-min ZHOU
2
;
Xin-yue FAN
2
;
Yu-quan ZHANG
2
;
Ting XIE
2
;
Xiao-qi LIN
2
;
Shan QIAO
1
;
Li-heng HAO
1
;
Meng WANG
1
;
Jian-ye ZHANG
2
Author Information
1. Department of Hepatobiliary Surgery, Bayannur Hospital, Bayannur 015000, China
2. Guangdong Key Laboratory of Molecular Target and Clinical Pharmacology, School of Pharmacy, Guangzhou Medical University, Guangzhou 511436, China
- Publication Type:Research Article
- Keywords:
resibufogenin;
human hepatocellular carcinoma;
italic>in vitro growth;
migration;
invasion
- From:
Acta Pharmaceutica Sinica
2022;57(7):2101-2107
- CountryChina
- Language:Chinese
-
Abstract:
This manuscript aims to investigate the effects of resibufogenin on the proliferation, migration and invasion of human hepatocellular carcinoma cells and its related mechanisms. MTT assay was used to determine the inhibitory effect of resibufogenin on the growth of four hepatocellular carcinoma cells in vitro. Wound-healing assay and Transwell assay were used to evaluate the migration and invasion ability of resibufogenin on MHCC-97H cells. Western blot assay was used to detect the expression of migration and invasion related proteins in MHCC-97H cells treated with different concentrations of resibufogenin. The results showed that resibufogenin significantly inhibited the proliferation of hepatocellular carcinoma cells in vitro. The half maximal inhibitory concentration (IC50) values on MHCC-97H, HepG2, SK-Hep-1 and Huh-7 cells were 0.55 ± 0.06, 2.83 ± 0.24, 5.25 ± 0.49, 14.89 ± 2.28 μmol·L-1, respectively. Resibufogenin also suppressed the migration and invasion of MHCC-97H cells in a concentration-dependent manner. The protein expression of integrin α2, integrin α6, integrin β1, N-cadherin, matrix metalloproteinase 2 (MMP2) and transcription factor Twist in MHCC-97H cells were decreased significantly with the increase of the concentration of resibufogenin, while the protein expression of E-cadherin increased. In addition, we found that p-PI3K/PI3K and p-AKT/AKT ratios were significantly reduced after treatment with resibufogenin. In conclusion, resibufogenin can inhibit the proliferation, migration and invasion of hepatocellular carcinoma MHCC-97H cells in vitro, which is related to the regulation of intracellular migration and invasion protein expression and PI3K/AKT signaling pathway.
