Evaluation of spike quality of Schizonepeta tenuifolia based on fingerprint and chemometrics
10.16438/j.0513-4870.2021-1850
- VernacularTitle:基于指纹图谱结合化学计量学方法评价荆芥穗质量
- Author:
Qun ZHANG
;
Sha LUO
;
Jie LI
;
Bei LI
;
Wen-yi NIU
;
Rui TAN
- Publication Type:Research Article
- Keywords:
italic>Schizonepeta tenuifolia;
HPLC;
fingerprint;
multicomponent determination;
chemical pattern recognition
- From:
Acta Pharmaceutica Sinica
2022;57(7):2146-2152
- CountryChina
- Language:Chinese
-
Abstract:
The quality control and evaluation methods of Schizonepeta tenuifolia were established by HPLC fingerprint, multi index component content determination and chemical pattern recognition to provide basis for the quality control of medicinal materials. The chemical components of 25 batches of Schizonepeta tenuifolia panicle medicinal materials and decoction pieces collected were analyzed by high performance liquid chromatography, and the common pattern of fingerprint was established. A total of 22 common chromatographic peaks were calibrated, and their similarity was more than 0.9. The samples were divided into three categories according to different producing areas by cluster analysis. The results of principal component analysis and cluster analysis were consistent. Finally, five differential markers of different batches of Schizonepeta tenuifolia were selected by orthogonal partial least squares discriminant analysis. Through the identification of the reference substance, it was determined that peak 9 was hesperidin, peak 10 was rosmarinic acid, peak 13 was tilianin, peak 14 was quercetin, and peak 20 was pulegone. The quality evaluation method established in this study is stable and reliable, and is suitable for the quality control of Schizonepeta tenuifolia.
