Effects of curcumin on memory follicular T cells in obese mice with colitis
10.16438/j.0513-4870.2021-1630
- VernacularTitle:姜黄素对肥胖小鼠溃疡性结肠炎记忆性滤泡性T细胞的调控作用
- Author:
Zeng-ping KANG
1
;
Hai-mei ZHAO
2
;
Meng-xue WANG
1
;
Jia-qi HUANG
1
;
You-bao ZHONG
1
,
3
;
Duan-yong LIU
4
Author Information
1. Graduate School, Jiangxi University of Chinese Medicine, Nanchang 330004, China
2. College of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, China
3. Experimental Animal Science and Technology Center, Jiangxi University of Chinese Medicine, Nanchang 330004, China
4. Formula-pattern Reasearch Center, Jiangxi University of Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
curcumin;
ulcerative colitis;
memory follicular T cell;
immunomodulation;
anti-inflammatory
- From:
Acta Pharmaceutica Sinica
2022;57(7):2068-2076
- CountryChina
- Language:Chinese
-
Abstract:
This study evaluated the regulatory effect of curcumin on memory follicular T cells (mTf) in obese mice with ulcerative colitis on the basis of determining its effective treatment of ulcerative colitis in obese mice. Forty male leptin mutant (ob/ob) mice were randomly divided into control group, control + curcumin group, dextran sodium sulfate (DSS) group and DSS + curcumin group, with 10 mice in each group. Mice in the DSS group and the DSS + curcumin group were induced by DSS to establish chronic ulcerative colitis model, and mice in the control + curcumin group and the DSS + curcumin group were given curcumin (200 mg·kg-1·d-1) by intragastric administration. Mice were sacrificed under anesthesia, and colon mass index, colon length and other conditions were observed in each group. Pathological injury of colonic was performed after HE staining. The levels of memory follicular helper T cells (mTfh) and memory follicular regulatory T cells (mTfr) in spleen of mice were detected by flow cytometry. The expression levels of interleukin-10 (IL-10) and interleukin-17A (IL-17A) in colon tissue were detected by ELISA. The results showed that curcumin significantly increased the body weight and colon length of obese mice with colitis, and decreased the colon weight, colon mass index and pathological score (P < 0.05). Curcumin significantly reduced the levels of central memory follicular T cells (cmTf), mTfh1, mTfh17 cells and the content of pro-inflammatory cytokine IL-17A (P < 0.01). The levels of effector memory follicular T cells (emTf) and mTfr and the content of anti-inflammatory cytokine IL-10 were increased (P < 0.05, P < 0.01). Therefore, curcumin may treat colitis in obese mice by regulating the balance of mTf cell subsets.
