Fagonia cretica: Identification of compounds in bioactive gradient high performance liquid chromatography fractions against multidrug resistant human gut pathogens
https://doi.org/10.47665/tb.39.2.006
- Author:
Tabassum, T.
1
;
Rahman, H.
1
;
Tawab, A.
2
;
Murad, W.
3
;
Hameed, H.
1
;
Shah, S.A.R.
1
;
Alzahrani, K.J.
4
;
Banjer, H.J.
4
;
Alshiekheid, M.A.
5
Author Information
1. Department of Microbiology, Abdul Wali Khan University Mardan, Pakistan
2. National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
3. Department of Botany, Abdul Wali Khan University Mardan, Pakistan
4. Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
5. Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia
- Publication Type:Journal Article
- Keywords:
F. cretica;
HPLC;
MDR;
MS-analysis.
- From:Tropical Biomedicine
2022;39(No.2):185-190
- CountryMalaysia
- Language:English
-
Abstract:
Plants are alternative source of natural medicines due to secondary active metabolites. Fagonia cretica
extracts and Gradient High-Pressure Liquid Chromatography fractionations were checked against
multidrug-resistant gastrointestinal pathogens including, Salmonella typhi, Escherichia coli and Shigella
flexneri. ESI-MS/MS analysis of bioactive HPLC fractions was performed to elucidate antibacterial
compounds. F. cretica extracts exhibited potential antibacterial activity. Twenty-four (24) HPLC fractions
were obtained from methanol, ethanol and aqueous extracts of F. cretica. Eighteen (18) fractions showed
antibacterial activity, while no activity was observed by the remaining six (6) fractions. HPLC fractions,
F1 (25g ± 0.20 mm) and F2 (15f
± 0.12 mm) of aqueous extract exhibited activity against multidrug
resistant GI pathogens. Gallic acid, quinic acid, cyclo-l-leu-l-pro, vidalenolone, liquirtigenin, rosmarinic
acid and cerebronic acid were identified in F1 fraction of aqueous extract, while succinic acid, cyclo (l-Leul-Pro) and liquirtigenin were identified in F2 fraction of aqueous extract through ESI-MS/MS analysis.
F. cretica extracts and HPLC fractions showed potential activity against MDR GI pathogens. Vidalenolone,
Cyclo-1-leu-1-pro and Cerebronic acid are first time reported in F. cretica. Further characterization of
bioactive compounds from F. cretica may be helpful to elucidate antibacterial therapeutic molecules.
- Full text:8.2022my1329.pdf
