Molecular characterisation and genetic affinities of Cyclophyllidean cestodes infecting wild rats in Peninsular Malaysia
https://doi.org/10.47665/tb.39.2.002
- Author:
Mohd-Saad, N.
1
;
Mohd Zain, S.N.
1
;
Sharma, R.S.K.
2
;
Omar, H.
1
,
3
Author Information
1. Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia&
2. Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
3. Museum of Zoology (Block J14), Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
Zoonoses;
Taeniidae;
Hymenolepididae;
genetic divergence;
murids.
- From:Tropical Biomedicine
2022;39(No.2):170-178
- CountryMalaysia
- Language:English
-
Abstract:
Cestode infections is widely reported in rodents, however species identification remains problematic due
to the genetic or interspecies variation. Therefore, this study was aimed to verify the Cyclophyllidean
parasites recovered from wild rats captured from different forest types using molecular based methods.
Maximum-likelihood (ML) and neighbour-joining (NJ) trees were constructed inferred from 18 small
subunit nuclear ribosomal RNA gene (18SrDNA) and mitochondrial cytochrome c oxidase subunit
one gene (COX1) sequences of cestode worms recovered from 124 individuals from four rat species.
Sequences obtained from both Hymenolepis diminuta and Hydatigera parva represents the first
records in Malaysia. All the sequences were successfully amplified with product with total length of
205 and 1202 base pairs (bp), respectively. Three cestode species from the Family Hymenolepididae
(Hymenolepis diminuta) and Family Taeniidae (Hydatigera parva; Hydatigera taeniaeformis) were
successfully characterized using phylogenetic analyses and haplotype networking. Phylogenetic
analysis showed that H. diminuta, Hydatigera parva (Hy. parva) and Hydatigera taeniaeformis (Hy.
taeniaeformis) formed its own monophyletic clade in 18SrDNA analyses. Results also showed that
Hy. taeniaeformis shared the same haplotype group with Hy. taeniaeformis from China (COX1) and
linked with Hy. taeniaeformis from Japan (18SrDNA) while the Malaysian H. diminuta clearly formed
a separate haplotype and networked with other regions. The Malaysian Hy. parva isolation, on the
other hand, appeared to be genetically distinct from the European Hy. parva (Spain) strain, but closely
linked to the local isolates. Molecular methods employed successfully improved in the detection of
complex species in this group. The findings showed that molecular data can be useful to deeply study
intra-specific variation in other cestode worms.
- Full text:8.2022my1327.pdf
