Content determination of five constituents in Shenfukang Ⅱ capsule by HPLC
- VernacularTitle:HPLC法测定肾复康Ⅱ号胶囊中5个成分的含量
- Author:
Jiyao YIN
1
;
Xia SHEN
1
;
Jing HU
2
;
Xiaomin CUI
2
;
Hui REN
2
;
Tong QU
2
;
Ning LI
2
;
Kai QU
1
,
3
;
Zhiyong CHEN
1
Author Information
1. College of Basic Medicine，Shaanxi University of Chinese Medicine，Shaanxi Xianyang 712083，China
2. Shaanxi Academy of Traditional Chinese Medicine，Xi’an 710003，China
3. Dept. Two of Nephropathy，Shaanxi Provincial Chinese Medicine Hospital，Xi’an 710003，China
- Publication Type:Journal Article
- Keywords:
Shenfukang Ⅱ capsule;
content determination;
HPLC method;
morroniside;
loganin;
paeoniflorin;
salvianolic
- From:
China Pharmacy
2022;33(15):1838-1841
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop an HPLC method for the simultaneous dete rmination of morroniside ，loganin，paeoniflorin， salvianolic acid B and icariin in Shenfukang Ⅱ capsule. METHODS The determination was performed on Agilent 5 TC-C18 column with mobile phase consisted of acetonitrile- 0.1％ phosphate acid （gradient elution ）at the flow rate of 1 mL/min. The column temperature was 30 ℃，and detection wavelength was set at 240 nm. The sample size was 10 μL. RESULTS The linear range of morroniside，loganin，paeoniflorin，salvianolic acid B and icariin were 4.80-240.00，4.84-242.00，7.00-350.00，4.72-236.00 and 5.18-259.00 μg/mL（r≥0.999 8），respectively. RSDs of precision ，stability and reproducibility tests were all lower than 3％（n＝6）. Average recoveries were 97.22％-101.36％ with the RSDs of 1.19％-2.43％（n＝6）. The contents of above 5 components in 5 batches of samples were 2.019 3-2.360 0，1.624 2-1.847 1，5.637 7-6.828 0，5.015 9-5.717 0 and 1.208 8-1.754 6 mg/g，respectively. CONCLUSIONS The method is simple ，accurate and reproducible. It can improve the quality control level of Shenfukang Ⅱ capsule.
