Evaluation of the quality of different germplasms of Rehmannia glutinosa based on iridoid glycosides
- VernacularTitle:基于环烯醚萜苷类成分评价不同种质地黄的质量
- Author:
Xiaotong GENG
1
;
Caixia XIE
2
;
Nan ZHANG
3
;
Jinping LI
4
;
Qiong CHEN
1
Author Information
1. School of Pharmaceutical Engineering，Xinyang Agriculture and Forestry University，Henan Xinyang 464000，China
2. College of Pharmacy，Henan University of Chinese Medicine，Zhengzhou 450000，China
3. Admissions Employment Office，Xinyang Normal University，Henan Xinyang 464000，China
4. Henan Xibanxia Pharmaceutical Company Limited，Henan Xinyang 464000，China
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
germplasms;
iridoid glycosides;
multivariate statistics;
quality evaluation
- From:
China Pharmacy
2022;33(15):1833-1837
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the quality of different germplasms of Rehmannia glutinosa based on iridoid glycosides. METHODS The contents of total iridoid glycosides ，catalpol，rehmaionoside D ，rehmaionoside A ，and leonuride in 18 batches of R. glutinosa from 6 germplasms（85-5，JinJiu，BX，BJ-1，Shandong，QH-1）were determined by ultraviolet spectrophotometry and high performance liquid chromatography. After normalization of the above content determination results ，the quality of different germplasm of R. glutinosa were evaluated by multiple statistical methods such as cluster analysis ，factor comprehensive analysis and partial least squares discriminant analysis （PLS-DA）. RESULTS Among 6 germplasms of R. glutinosa ，the content of total iridoid glycosides in R. glutinosa 85-5 was the highest ，and the content of catalpol in R. glutinosa BX was the highest ；the contents of rehmannioside D and rehmannioside A in R. glutinosa JinJiu were the highest ，and the content of leonuride in R. glutinosa BX was the highest. Cluster analysis showed that R. glutinosa JinJiu were clustered into one category ，R. glutinosa BX clustered into one category ，R. glutinosa Shandong and R. glutinosa BJ-1 were clustered into one category ，and R. glutinosa QH-1 and 85-5 were clustered into one category. Through factor comprehensive analysis ，there were differences in the quality of different germplasms of R. glutinosa . The comprehensive score of R. glutinosa BX，Shandong，85-5，BJ-1，QH-1，JinJiu were 2.283 9，1.689 1，1.664 8， 1.503 3，1.469 0，1.214 6，respectively. PLS-DA showed that variable importance projection value of total iridoid glycosides ， catalpol and leonuride were all higher than 1. CONCLUSIONS The quality difference of R. glutinosa from different germplasms may be caused by total iridoid glycosides ，catalpol and leonuride.
