Relationship between hepatitis B virus genotyping and primary hepatocellular carcinoma of 182 patients
10.3969/j.issn.1006-2483.2022.04.033
- VernacularTitle:182例患者乙肝病毒基因分型与原发性肝癌的关系
- Author:
Xin WANG
1
,
2
;
Le SONG
3
;
Xin FANG
2
;
Bicheng ZHANG
4
,
5
Author Information
1. Wuhan University , Wuhan , Hubei 430071 , China
2. Department of Infection , Wuhan Yaxin General Hospital , Wuhan , Hubei 430010 , China
3. Department of Infection , PLA Central Theater General Hospital , Wuhan , Hubei 430070 , China
4. Cancer Center , Wuhan University People'
5. s Hospital , Wuhan , Hubei 430060 , China
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus genotyping;
Primary liver cancer;
Mutation rate;
High-risk factors
- From:
Journal of Public Health and Preventive Medicine
2022;33(4):141-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between hepatitis B virus genotyping and primary liver cancer (PHC) in Wuhan, and to provide a theoretical basis for the early prevention and diagnosis of PHC. Methods Patients with chronic hepatitis B (CHB) from Wuhan Sub-heart General Hospital for treatment from February 2020 to February 2021 were selected and divided into PHC group (182 cases) and control group (189 cases) according to whether they were complicated with primary liver cancer. 5ml of fasting elbow venous blood was taken from all subjects at admission. HBV genotyping was determined by real-time fluorescence quantitative PCR. The DNA of CHB virus was determined by fluorescence probe hybridization and PCR amplification, and genotyping and drug-resistant mutation points were detected according to the product sequencing analysis. Spearman linear correlation analysis was used to analyze the correlation between genotyping and mutation rate of PHC patients. Results The proportion of C genotype in PHC group was significantly higher than that in non-PHC group (P<0.05). There was no significant difference in the proportion of B genotype and mixed B and C genotype between 2 groups (P>0.05). The proportion of HEPATITIS B virus mutation in PHC group (114/182) was significantly higher than that in control group (84/189) (χ2=12.331, P<0.05). There was no significant difference in the proportion of HBV B type and B, C mixed mutation between the two groups (P>0.05). The proportion of HBV C mutant in PHC group was significantly higher than that in control group (P<0.05). The proportion of HBV C mutation in PHC group was significantly higher than that of HBV B and B, C mixed type (P<0.05). Spearman correlation analysis showed that the mutation rate of C genotype and C genotype was positively correlated with the occurrence of PHC, and the correlation coefficients were (r1=0.349, r2=0.305, P<0.05). Conclusion The HBV genotype of PHC patients is mainly TYPE C, and has a high mutation rate of C genotype. It can be used for diagnosis of PHC by detecting the genotyping of CHB and mutation rate of C genotype in clinic.
