Potential effect of methanol leaf extracts from three Podocarpus species on experimental cryptosporidiosis
https://doi.org/10.47665/tb.39.1.009
- Author:
El-Hawary, S.S.
1
;
Kirollos, F.N.
1
;
Taha, K.F.
2
;
Dahab, A.A.
3
;
El-Mahis, A.A.
2
;
El-Sayed, S.H.
4
Author Information
1. Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
2. Applied Research Center of Medicinal Plants, National Organization of Drug Control and Research, Cairo, Egypt
3. Department of Medicinal and Aromatic Plants, Horticulture Research Institute, ARC, Cairo, Egypt
4. Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, Egypt
- Publication Type:Journal Article
- Keywords:
Cryptosporidium parvum;
Podocarpus;
Nitazoxanide;
mice;
colorimetric assay.
- From:Tropical Biomedicine
2022;39(No.1):99-107
- CountryMalaysia
- Language:English
-
Abstract:
Cryptosporidiosis causes diarrhea in both immunocompetent and immunocompromised
individuals, with acute manifestations occurring particularly in children and the elderly. Up
till now, there is no curative therapy for cryptosporidiosis, so discovery of new classes of
drugs are of great importance. This study aimed to examine the effect of methanol leaves
extracts of the three Podocarpus species; P. macrophyllus (Thunb.), P. gracilior (Pilg.) and P.
elongatus (Aiton) L’ Hér. ex Pers and their combination on Cryptosporidium parvum (C. parvum) in
experimentally infected mice in comparison with the commercially used drug, Nitazoxanide.
As well as spectrophotometric estimation of the total phenolic and flavonoid content of
these extracts was done. Results revealed that treatment with these three Podocarpus extracts
and their combination showed a significant reduction of the number of C. parvum oocyst
shed in the stool of infected mice compared to infected control group and Nitazoxanideinfected treated group at P < 0.001. The combination of the three Podocarpus extracts was the
most effective treatment showing the lowest number of oocysts shedding in comparison
with other used extracts and Nitazoxanide. Histopathological inspection of sections from
ilium and colon displayed signs of improvement after treatment with P. macrophyllus and P.
gracilior extracts and more remarkable improvement when the three extracts were combined.
It was concluded that the three Podocarpus species extracts used in this study had a promising
anti-Cryptosporidium activity especially when they were combined.
- Full text:8.2022my1319.pdf
