Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells
10.12016/j.issn.2096-1456.2022.11.003
- Author:
DENG Zilong
1
;
YAN Wenjuan
1
;
ZHAO Wanghong
2
;
WU Buling
3
Author Information
1. 1.Department of Stomatology, Nanfang Hospital, Southern Medical University 2College of Stomatology, Southern Medical University.
2. 1.Department of Stomatology, Nanfang Hospital, Southern Medical University 2.College of Stomatology, Southern Medical University
3. 1.College of Stomatology, Southern Medical University 2.Department of Operative Dentistry and Endodontics, Shenzhen Stomatology Hospital (Pingshan), Southern Medical University
- Publication Type:Journal Article
- Keywords:
dental pulp stem cells;
proliferation;
migration;
N-cadherin;
dental pulp regeneration;
cell cycle;
cell apoptosis;
lentivirus;
microenvironment
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2022;30(11):779-784
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells (DPSCs) and to provide experimental evidence for DPSCs-based dental pulp regeneration.
Methods: DPSCs were transfected with N-cadherin shRNA lentivirus, and the knockdown efficiency of N-cadherin at both the mRNA and protein levels was confirmed by qRT-PCR and Western blot. The experiment included a negative control group (shRNA -NC) and an N-cadherin shRNA silencing group. Cell proliferation was detected by the CCK-8 method. Cell cycle and apoptosis were assessed by flow cytometry, and cell migration was detected using the Transwell method.
Results:N-cadherin shRNA significantly reduced the expression levels of N-cadherin mRNA and protein in DPSCs (P<0.001). The proliferation activity of the N-cadherin shRNA group was significantly greater than that of the shRNA-NC group on the 3rd and 4th days after cell inoculation and lower than that of the shRNA-NC group from the 6th to 8th days (P<0.05). On the 3rd day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.05). On the 6th day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was lower than that in the shRNA-NC group (P<0.05), and the proportion of apoptotic cells in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.01). Low densities cells and high densities cells were inoculated into Transwell upper chamber for 20 h, the number of cells passing through the membrane pores of upper chamber in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.001).
Conclusion:Silencing N-cadherin expression can promote the early proliferation and migration of DPSCs.
