Antibacterial activity of plant essential oils against indigenously characterized methicillin-resistant Staphylococcus aureus (MRSA)
https://doi.org/10.47665/tb.39.1.005
- Author:
Ali, T.
1
;
Anjum, A.A.
1
;
Sattar, M.M.K.
2
;
Ali, M.A.
1
;
Kamran, M.
3
;
Tariq, M.
1
;
Manzoor, R.
1
Author Information
1. Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan (54000)
2. Department of Microbiology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan (63100)
3. Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan (46000)
- Publication Type:Journal Article
- Keywords:
Essential oil;
Eucalyptus globules;
methicillin resistance;
n-hexane;
Staphylococcus aureus.
- From:Tropical Biomedicine
2022;39(No.1):17-25
- CountryMalaysia
- Language:English
-
Abstract:
Plant essential oils were evaluated for antimicrobial activity against Methicillin-resistant
Staphylococcus aureus (MRSA). The isolates (n=03) were procured from Institute of Microbiology,
UVAS Lahore, Pakistan. After biochemical and 16S rRNA gene-based PCR characterization,
accession numbers were retrieved from NCBI i.e. MW344063.1, MW344064.1 and MW344065.1.
These isolates exhibited molecular positivity by multiplex PCR for mecA, coa and eta toxin
genes. Moreover, these isolates exhibited resistance to cefoxitin, ampicillin, amoxicillin,
penicillin, amoxicillin clavulanate, ciprofloxacin, erythromycin and gentamicin. The antibiotic
resistant isolates were evaluated for antimicrobial activity of plant essential oils. The
highest zone of inhibition (mean ZOI±S.D.) was measured for Cinnamomum verum (22.67±1.52
mm) followed by Eucalyptus globulus (18.67±2.51 mm) and Syzygium aromaticum (12.67±2.51 mm).
Lowest mean MIC value (0.33±0.11 mg/mL) was recorded for E. globulus. Eucalyptus globulus was
processed for fractionation by column chromatography and n-hexane, chloroform, n-hexane
+ chloroform and ethyl-acetate fractions were evaluated for antibacterial activity. Lowest
mean MIC (10.04±5.80 mg/mL) was recorded for E. globulus n-hexane fraction. Cell survival
percentage of BHK21 cell line was 51.7% at 54.87mg/mL concentration of E. globulus n-hexane
fraction. Through gas chromatography-mass spectrometry (GC-MS) analysis of n-hexane
fraction, benzene was found abundant (29.9%) as active compound. It was concluded that E.
globulus n-hexane fraction exhibited significantly promising results against MRSA.
- Full text:8.2022my1308.pdf
