Effects of Guhong Injection on Motor Dysfunction in Cerebral Ischemia-reperfusion Rats
10.3969/j.issn.1006-9771.2015.01.004
- VernacularTitle:谷红注射液对脑缺血再灌注损伤大鼠运动功能障碍的影响
- Author:
Rui ZHANG
;
Siyuan FAN
;
Zhen LIANG
;
Chao JI
;
Nan YANG
;
Yanyong LIU
;
Liying CUI
;
Pingping ZUO
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion, Guhong Injection, motor dysfunction, substantia nigra, tyrosine hydroxylase
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of Guhong Injection on motor dysfunction in rats after cerebral ischemia- reperfusion. Methods The middle cerebral arteries were occluded for 2 hours and re-perfused in Sprague-Dawley rats. They were divided in sham group, model group, Aceglutamide group, Safflowere group and Guhong group, which were intravenously administrated with normal saline, Aceglutamide, Safflower or Guhong 24 hours after operation, and continued for 14 days. They were tested with the beam-walking test after treatment. Tyrosine hydroxylase (TH) immunohistochemical staining was used to investigate the viability of neurons in the substantia nigra. Results The model group spent more time in the beam-walking test than that in the sham group (P<0.01), and it decreased in the Safflower group and Guhong group compared with that in the model group (P<0.05). The TH-positive neurons decreased in the model rat compared with that in the sham group (P<0.001), and increased in both Safflower and Guhong groups compared with that in the model group (P<0.01). Conclusion Guhong administration could significantly improve the motor dysfunction in rats after cerebral ischemia- reperfusion, which might be related to provent the neurons from injury in the substantia nigra.
