Effects of Morroniside on Expression of Matrix Metalloproteinases in Peri- infarct Cortex after Cerebral Ischemia- reperfusion in Rats
10.3969/j.issn.1006-9771.2015.01.002
- VernacularTitle:莫诺苷对脑缺血再灌注大鼠皮层梗死周边区基质金属蛋白酶表达的影响
- Author:
Hongli HOU
;
Fangling SUN
;
Houxi AI
;
Li ZHANG
;
Wen WANG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion, morroniside, matrix metalloproteinase-2, matrix metalloproteinase-9, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of morroniside on the expression of matrix metalloproteinase (MMP) -2 and MMP-9 in the peri- infarct cortex 3 days after cerebral ischemia- reperfusion. Methods 15 male Sprague-Dawley rats were randomly divided into sham group (n=3), ischemia group (n=3), and morroniside groups (low, medium and high dosage groups, n=3). The middle cerebral artery were occluded for 30 min, and re-perfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of MMP-2 and MMP-9 in peri-infarct cortex were detected with immunohistochemistry staining 3 days after operation. Results The expression of MMP-2 and MMP-9 increased in the ischemia group compared with the sham group (P<0.01), and it decreased in all the morroniside groups compared with the ischemia group (P<0.01). Conclusion Morroniside could decrease the expression of MMP-2 and MMP-9 in the peri-infarct cortex 3 days after ischemia, suggesting protecting the function of blood-brain barrier from ischemia.
