Evaluation of commercial serological assays in Malaysia for detection of anti-Zika virus antibodies
https://doi.org/10.47665/tb.38.4.105
- Author:
Khoo, H.Y.
1
;
Lee, H.Y.
1
;
Khor, C.S.
1
;
Tan, K.K.
1
;
AbuBakar, S.
1
,
2
,
3
Author Information
1. Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Centre of Excellence (HICoE), Universiti Malaya, 50603 Kuala Lumpur, Malaysia&
2. WHO Collaboration Centre for Arbovirus Reference and Research Malaysia, Universiti Malaya, 50603 Kuala Lumpur, Malaysia (MAA-12)&
3. Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
Infectious diseases;
vector-borne diseases;
antibodies;
Zika virus;
ELISA
- From:Tropical Biomedicine
2021;38(No.4):613-621
- CountryMalaysia
- Language:English
-
Abstract:
The recommended test guidelines for Zika virus (ZIKV) include using both molecular and
serological tools. While the molecular tools are useful for detecting acute infection, the
serological tools are useful for the detection of previous infections. Nevertheless, detection
of ZIKV-specific antibodies remains a challenge due to the high cross-reactivity between
ZIKV and other flaviviruses such as dengue virus (DENV) and Japanese encephalitis virus
(JEV). The objective of this study is to evaluate the commercially available enzyme-linked
immunosorbent assay (ELISA) for the detection of ZIKV IgG. In this study, we evaluated 6
commercially available anti-ZIKV IgG ELISA kits. Pre-characterized serum panels consisting
of 70 sera were selected for the evaluation. The diagnostic accuracy of each ELISA kits was
determined and compared to the gold standard, Foci Reduction Neutralization Test (FRNT).
The present study established that the performance of the NS1-based anti-ZIKV IgG ELISA kit
was superior to that which uses of the E protein as antigen. Overall, commercial ZIKV IgG
ELISA showed varying test performances, with some achieving moderate to high test
sensitivities and specificities. When compared against the FRNT, the test sensitivities ranged
from 7.1% to 78.6%, whereas, the test specificities ranged from 40.0% to 100%. Limitation to
the study includes the cross reactivity between flavivirus and specificity of the kit in
addressing the cross reactivity.
- Full text:8.2021my1305.pdf
