TSPAN8 is involved in lipid metabolism in non-alcoholic fatty liver disease in mice.
10.12122/j.issn.1673-4254.2022.05.11
- Author:
Jia ZHANG
1
;
Wei XUE
1
;
Shu Jun ZHANG
1
;
Ya Li ZHU
1
;
Cheng YANG
1
;
Yue GAO
1
;
Ling Feng SHI
1
;
Wen Xiang HUANG
1
Author Information
1. Department of Infectious Diseases, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
lipid metabolism;
non-alcoholic fatty liver disease;
tetraspanin 8
- MeSH:
Animals;
Diet, High-Fat/adverse effects*;
Fatty Acids, Nonesterified;
Lipid Metabolism;
Liver/metabolism*;
Male;
Mice;
Mice, Inbred C57BL;
Non-alcoholic Fatty Liver Disease/metabolism*;
RNA, Messenger/metabolism*
- From:
Journal of Southern Medical University
2022;42(5):705-711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of tetraspanin 8 (TSPAN8) expression levels and its role in lipid metabolism during the development of non-alcoholic fatty liver disease (NAFLD).
METHODS:Thirty male C57BL/6J mice were randomly divided into normal diet group and high-fat diet (HFD) group (n=15), and after feeding for 1, 3, and 6 months, the expression levels of TSPAN8 in the liver tissues of the mice were detected with Western blotting. In a HepG2 cell model of NAFLD induced by free fatty acids (FFA), the effect of TSPAN8 overexpression on lipid accumulation was examined using Oil Red O staining and an automated biochemical analyzer, and the mRNA expressions of the key genes involved in lipid metabolism were detected using qRT-PCR.
RESULTS:Western blotting showed that compared with that in mice with normal feeding, the expression of TSPAN8 was significantly decreased in the liver tissues of mice with HFD feeding for 3 and 6 months (P < 0.05). In HepG2 cells, treatment with FFA significantly decreased the expression of TSPAN8 at both the mRNA and protein levels (P < 0.01). TSPAN8 overexpression in FFA-treated cells showed significantly lowered intracellular triglyceride levels (P < 0.001) and obviously reduced mRNA expression of fatty acid transport protein 5 (FATP5) (P < 0.01). The expression of FATP5 was significantly increased in FFA-treated cells as compared with the control cells (P < 0.001).
CONCLUSION:TSPAN8 is involved in lipid metabolism in NAFLD, and overexpression of TSPAN8 may inhibit cellular lipid deposition by reducing the expression of FATP5.
