Agkistrodon halys venom antitumor component-I inhibits vasculogenic mimicry in triple-negative breast cancer cells in vitro by down-regulating MMP2.
10.12122/j.issn.1673-4254.2022.03.18
- Author:
Yu GE
1
;
Lin Ming LU
1
;
Shu Yu TIAN
1
;
Yu XIAO
1
;
Shang Fu XIE
1
;
Qi WANG
1
;
Hui ZHI
1
Author Information
1. Department of Pathology, Wannan Medical College, Wuhu 241002, China.
- Publication Type:Journal Article
- Keywords:
agkistrodon halys venom antitumor component-I;
matrix metalloproteinase-2;
triple-negative breast cancer;
vasculogenic mimicry
- MeSH:
Agkistrodon/metabolism*;
Animals;
Cell Line, Tumor;
Healthy Life Expectancy;
Humans;
Matrix Metalloproteinase 2/metabolism*;
Neovascularization, Pathologic/metabolism*;
Triple Negative Breast Neoplasms/metabolism*;
Venoms
- From:
Journal of Southern Medical University
2022;42(3):438-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effect of agkistrodon halys venom antitumor component-I (AHVAC-I) on vasculogenic mimicry (VM) formation in triple-negative breast cancer MDA-MB-231 cells and explore its possible mechanism.
METHODS:CCK8 assay was used to determine the optimal concentration of AHVAC-I for cell treatment based on its halfinhibitory concentration (IC50). MDA-MB-231 cells were treated with different concentrations of AHVAC-I or 5-Fu, and the changes in vasomimetic capacity of the cells were examined using Matrigel assay. The expression levels of matrix metalloproteinase-2 (MMP2) and MMP9 in the treated cells were detected using quantitative PCR and Western blotting.
RESULTS:Compared with the control treatment with culture medium, treatment with 5, 10 and 20 μg/mL AHVAC-I significantly reduced vasomimetic ability of MDA-MB-231 cells in a dose-dependent manner (P < 0.01). MMP2 supplementation obviously restored the vasomimetic ability of the cells inhibited by AHVAC-I.
CONCLUSION:AHVAC-I inhibits VM formation in triplenegative breast cancer cells in vitro by down-regulating MMP2 production.
