Role of CaMK II in pancreatic injury in mice with severe acute pancreatitis.
10.12122/j.issn.1673-4254.2022.02.17
- Author:
Wen JIANG
1
;
Jun WU
1
;
Jia Rong ZENG
1
;
Guang Xu JING
1
;
Li Jun TANG
1
;
Hong Yu SUN
1
Author Information
1. College of Medicine Southwest Jiaotong University, Chengdu 610063, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
CaMKⅡ;
KN93;
nuclear factor-κB;
severe acute pancreatitis
- MeSH:
Acute Disease;
Animals;
Inflammation/metabolism*;
Male;
Mice;
NF-kappa B/metabolism*;
Pancreas/pathology*;
Pancreatitis/pathology*
- From:
Journal of Southern Medical University
2022;42(2):286-292
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of Ca2+/calmodulin-dependent protein kinase II (CaMK Ⅱ) in pancreatic tissues of mice with severe acute pancreatitis (SAP) and explore the protective effect of KN93, a CaMK Ⅱ inhibitor, against pancreatic injury in SAP and the possible mechanism.
METHODS:Thirty-six healthy male C57 mice were randomly divided into sham operation group, SAP group, KN93 group and SAP + KN93 group (n=9). Serum and pancreatic tissue samples were collected 24 h after modeling. The pathological changes in the pancreatic tissues were observed using HE staining. Serum lipase and amylase activities and the levels of inflammatory factors were detected using ELISA. Western blotting was used to detect the expressions of CaMK Ⅱ, p-CaMK Ⅱ, p-NF-κB, MAPK and p-MAPK in mouse pancreas.
RESULTS:Compared with those in sham operation group, the expressions of p-CaMK Ⅱ, p-NF-κB and p-MAPK were significantly increased in SAP group (P < 0.05). KN93 treatment obviously alleviated pathological injuries of the pancreas in SAP mice, and significantly lowered serum levels of lipase, amylase and inflammatory factors (TNF-α and IL-6) and phosphorylation levels of NF-κB, ERK and MAPK proteins (P < 0.05).
CONCLUSION:The activity of CaMK Ⅱ is significantly increased in the pancreatic tissue of SAP mice. KN93 can alleviate pancreatic injury and inflammation in SAP mice possibly through the ERK/MAPK signaling pathway.
