Inhibiting ferroptosis attenuates myocardial injury in septic mice: the role of lipocalin-2.
10.12122/j.issn.1673-4254.2022.02.13
- Author:
Yu Hui HUANG
1
;
Gong Peng ZHANG
2
;
Huan LIANG
1
;
Zhen Zhen CAO
3
;
Hong Wei YE
1
;
Qin GAO
1
Author Information
1. Department of Physiology, Bengbu Medical College, Bengbu 233000, China.
2. Department of Clinical Medicine, Bengbu Medical College, Bengbu 233000, China.
3. Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
ferroptosis;
ferrostatin-1;
lipocalin-2;
myocardial injury;
sepsis
- MeSH:
Animals;
Ferroptosis;
Heart Injuries;
Lipocalin-2;
Male;
Mice;
Mice, Inbred C57BL;
Sepsis/metabolism*
- From:
Journal of Southern Medical University
2022;42(2):256-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the contribution of ferroptosis to myocardial injury in mouse models of sepsis and the role lipocalin-2 (Lcn2) in ferroptosis.
METHODS:Adult male C57BL/6 mice were randomized equally into sham-operated group, cecal ligation and puncture (CLP)-induced sepsis group, and CLP + Fer-1 group where the mice received intraperitoneal injection of 5 mg/mL Fer-1 (5 mg/kg) 1 h before CLP. The left ventricular functions (including LVEF%, LVFS%, LVIDd and LVIDs) of the mice were assessed by echocardiography at 24 h after CLP. Myocardial injury in the mice was observed with HE staining, and the changes of myocardial ultrastructure and mitochondria were observed using transmission electron microscopy (TEM). Serum TNF-α level was measured with ELISA, and the changes of myocardial iron content were detected using tissue iron kit. The protein expressions of myocardial Lcn2, glutathione peroxidase 4 (GPX4) and ferroptosis suppressor protein 1 (FSP1) were determined with Western blotting.
RESULTS:The septic mice showed significantly decreased LVEF%, LVFS% and LVIDd and increased LVIDs at 24 h after CLP (P < 0.05), and these changes were significantly improved by Fer-1 treatment. Sepsis caused obvious myocardial pathologies and changes in myocardial ultrastructure and mitochondria, which were significantly improved by Fer-1 treatment. Fer-1 treatment also significantly ameliorated sepsis-induced elevations of serum TNF-α level, myocardial tissue iron content, and Lcn2 protein expression and the reduction of GPX4 and FSP1 protein expression levels (P < 0.05).
CONCLUSION:GPX4- and FSP1-mediated ferroptosis are involved in myocardial injury in mice with CLP-induced sepsis, and inhibition of ferroptosis can attenuate septic myocardial injury, in which Lcn2 may play a role.
