Transforming growth factor-β1 induces transformation of rat meningeal fibroblasts into myofibroblasts by upregulating Shh signaling.
10.12122/j.issn.1673-4254.2022.02.12
- Author:
Jun WEN
1
;
Hui Min ZHU
1
;
Xue Mei LI
1
;
Jia Gui HUANG
1
;
Yue CHEN
1
;
Qin YANG
1
Author Information
1. Department of Neurology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Journal Article
- Keywords:
Shh signaling;
fibroblasts;
myofibroblasts;
transforming growth factor-β1
- MeSH:
Animals;
Fibroblasts/metabolism*;
Hedgehog Proteins;
Myofibroblasts/metabolism*;
Rats;
Rats, Sprague-Dawley;
Transforming Growth Factor beta1/metabolism*
- From:
Journal of Southern Medical University
2022;42(2):250-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of TGF-β1 on Shh signaling pathway during the transformation of meningeal fibroblasts into myofibroblasts.
METHODS:Primary meningeal fibroblasts were isolated from neonatal (24 h) SD rats and purified using type Ⅳ collagenase. The isolated cells were treated with 10 ng/mL TGF-β1 alone or in combination with 20 μmol/L SB-431542 (a TGF-β1 receptor inhibitor) for 72 h, and the changes in proliferation and migration abilities of the fibroblasts were assessed with CCK-8 assay and cell scratch test. The expression of fibronectin (Fn) was detected with immunofluorescence assay, and Western blotting was performed to examine the expressions of Fn, α-SMA and Shh protein in the cells; the expression of Shh mRNA was detected with real-time fluorescence quantitative PCR.
RESULTS:TGF-β1 treatment obviously enhanced the proliferation and migration of primary meningeal fibroblasts (P < 0.05), and promoted the transformation of meningeal fibroblasts into myofibroblasts and the secretion of Fn (P < 0.05). TGF-β1 treatment also upregulated the expression of Shh at both protein and mRNA levels (P < 0.05). Treatment with SB-431542 partially blocked the effect of TGF-β1 on the transformation of meningeal fibroblasts (P < 0.05).
CONCLUSION:TGF-β1 can induce the transformation of meningeal fibroblasts into myofibroblasts by up-regulating Shh expression in Sonic Hedgehog signaling pathway.
