Stable study of gene sequence of seed lot system G1P4 (2001019210) during production of rotavirus vaccine
- Author:
Luan Thi Le
;
Hien Dang Nguyen
;
Huong Thi Mai Nguyen
- Publication Type:Journal Article
- Keywords:
rotavirus vaccine;
gene sequence
- MeSH:
rotavirus vaccine;
gene sequence
- From:Journal of Preventive Medicine
2008;18(6):5-11
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Presently, toxicity decreased oral live rotavirus is a candidate for vaccine for the prevention of rotavirus induced diarrhea. According to the World Health Organization, the seed lot system is robustly checked, in which determining the stable of gene sequence. Objective: To determine the sequence of genes 4: 6: 9: 10 with base pair correlative 855: 824: 1314: 734 of seed lot system G1P4 (2001019210) during production of rotavirus vaccine. Subject and methods: Gene 4 (VP4), gene 6 (VP6), gene 9 (VP7) and gene 10 (NSP4) of seed lot system G1P4 were determined for gene sequencing and then comparing the nucleotide sequence as well as deduced amino acids from original strain with the produced strain and vaccine virus. Results and Conclusion: There was no different for nucleotide and deduced amino acid sequence from the original strain during the production of rotavirus vaccine of G1P4 MS (2001019210) to producing strains of G1P4 WS and vaccine strains of G1P4 VX.
