Determination of acetochlor and its metabolites in urine using liquid chromatography-tandem mass spectrometry.
10.3760/cma.j.cn121094-20210917-00464
- Author:
Meng Yao CHEN
1
;
Hao ZHANG
2
;
Zong Li HUO
2
;
Hao CHEN
1
;
Jian Rui DOU
3
;
Bao Li ZHU
4
;
Feng ZHANG
5
Author Information
1. School of Public Health, Nanjing Medical University, Nanjing, 211166, China.
2. Physical and Chemical Inspection Institute, Jiangsu Provincial Center for Disease Control and Prevention, Jiangsu, 210028, China.
3. Department of Occupational Health, Yangzhou Center for Disease Control and Prevention, Yangzhou, 225000, China.
4. School of Public Health, Nanjing Medical University, Nanjing, 211166, China Occupational Disease Prevention and Control Institute, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210028.
5. Technical Service Department, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210028.
- Publication Type:Journal Article
- Keywords:
Acetochlor;
LC-MS/MS;
Metabolites;
Solid phase extraction;
Urine
- MeSH:
Acetonitriles;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Humans;
Solid Phase Extraction;
Tandem Mass Spectrometry;
Toluidines
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(5):373-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A method for the determination of acetochlor and its metabolites in urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established. Methods: After cleaned-up by a HLB extraction cartridges, the urine was eluted with 1% acetic acid acetonitrile solution. The target compounds were separated by ACQUITY UPLC®HSS T3 Column (2.1 mm×100 mm×1.8 μm) by using 1% formic acid solution and acetonitrile as mobile phase with gradient elution program, and analyzed in positive electrospray ionization mode by liquid chromatography tandem mass spectrometry. Results: All the target compounds showed good linear relationships in the range of 1-50 μg/L, and the correlation coefficients (r) were higher than 0.997. The recoveries rates at three different spiked levels for all target compounds in blank matrices were 107.6%-129.1%, and the relative standard deviations (RSD) were 1.5%-9.9% (n=6) . The limits of detection and quantitation of the method were 0.04-0.11 μg/L and 0.15-0.42 μg/L, respectively, and target substances were detected in all urine samples from occupational exposure workers to acetochlor. Conclusion: This method is suitable for rapid screening and analysis of acetochlor and metabolites in urine with the advantages of accuracy, rapidity, simplicity, high sensitivity and good specificity.
