Study on determination of 2-thioxothiazolidine-4-carboxylic Acid in urine by high performance liquid chromatography.
10.3760/cma.j.cn121094-20210129-00061
- Author:
Jin Feng JIANG
1
;
Wei Li SONG
1
;
Ya Pan LIU
1
;
Jun Pin LIU
1
;
Min WANG
1
Author Information
1. Department of Health Laboratory Technology, Wuhan Prevention and Treatment Center for Occupational Disease, Wuhan 430015, China.
- Publication Type:Journal Article
- Keywords:
2-Thioxothiazolidine-4-carboxylic acid;
Carbon disulfide;
Chromatography, high performance liquid;
Urine
- MeSH:
Carbon Disulfide;
Chromatography, High Pressure Liquid/methods*;
Humans;
Thiazoles/urine*;
Thiazolidines;
Thiones
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(5):370-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a high performance liquid chromatography method for the determination of 2-thioxothiazolidine-4-carboxylic acid (TTCA) in urine. Methods: After acidification with hydrochloric acid, TTCA in urine was first extracted by ethyl acetate with excessive sodium chloride, then gradient separated by a symmetry C18 column and then detected by a diode array detector. The quantification was based on a working curve of external standard method. Results: The linear relationship of TTCA in urine was good in the range of 0.03-10.00 mg/L, and the correlation coefficient was 0.9999. The detection limit and minimum quantitative concentration of TTCA in urine were 0.008 mg/L and 0.027 mg/L. The intra-assay precision of the method was 0.9%-1.4%, the inter-assay precision was 1.3%-3.5%, and the average recovery was 85.0%-92.7% while the concentrations of TTCA in urine was 0.8, 2.0 and 8.0 mg/L, respectively (n=6) . Conclusion: The gradient elution high performance liquid chromatography method has simple operation and high sensitivity, and it is suitable for the determination of TTCA on a low level in urine for occupational workers exposure to carbon disulfide.
