Determination of creatinine and 2-thiothiazolidine-4-carboxylic acid in urine by ultra high performance liquid chromatography tandem mass spectrometry.
10.3760/cma.j.cn121094-20210817-00399
- VernacularTitle:尿中肌酐和2-硫代噻唑烷-4-羧酸测定的超高效液相色谱串联质谱法
- Author:
Xiao Yun XIONG
1
;
Qiu Xia JIANG
1
;
Wei WANG
1
;
Shan Ci MAO
1
Author Information
1. Jiangsu Provincial Center for Disease Control and Prevention, Occupational Disease Prevention and Control Institute, Nanjing 210028, China.
- Publication Type:Journal Article
- Keywords:
2-thiothiazolidine-4-carboxylic acid;
Carbon disulfide;
Creatinine;
Liquid chromatography tandem mass spectrometry;
Urine
- MeSH:
Chromatography, High Pressure Liquid;
Creatinine;
Humans;
Tandem Mass Spectrometry;
Thiazolidines
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(3):226-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an ultrahigh performance liquid chromatography tandem mass spectrometry method for the determination of creatinine (Cre) and 2-thiothiazolidine-4-carboxylic acid (TTCA) in urine. Methods: In October 2020, the end-of-shift urine samples of the monitored subjects were taken, and the filtrate was prepared by centrifugation. After separated by ultra high performance liquid chromatography C18 column, acetonitrile and 0.2% acetic acid aqueous solution were used as mobile phases for gradient elution, the three quadrupole tandem mass spectrometry adopted an electrospray ion source (ESI) , the ion source temperature was 500 ℃ , and the air curtain gas flow rate was 31.4 L/min, qualitative and quantitative analysis of Cre and TTCA were carried out under the multiple reaction monitoring mode. Results: The linear range of Cre was 1.0-1 000.0 μg/L, the linear equation was y=947.3x-1605.6, and the correlation coefficient was 0.9994. The detection limit and the limit of quantitation were 0.3, 1.0 μg/L. When the addition concentrations were 50.0, 150.0 and 450.0 μg/L, the recovery rates were 92.8%-94.6% , the intra assay precisions were 3.6%-5.7% , and the inter assay precisions were 3.4%-5.4%. The linear range of TTCA was 0.1-200.0 μg/L, the linear equation was y=1164.7x-2243.9, and the correlation coefficient was 0.9991. The detection limit and the limit of quantitation were 0.03, 0.1 μg/L. When the addition concentrations were 10.0, 40.0 and 160.0 μg/L, the recovery rates were 90.8%-93.6%, the intra assay precisions were 4.6%-7.4%, and the inter assay precisions were 4.4%-6.9%. Conclusion: The sample pretreatment process of the ultra high performance liquid chromatography tandem mass spectrometry method for the determination of Cre and TTCA in urine is simple, and the continuous determination of Cre and TTCA in urine can be realized only by switching mass spectrometry parameters under the same chromatographic conditions, which is accurate and efficient, and each performance index of the method meets the determination requirements.
