Fast determination of bongkrekic acid in plasma by high performance liquid chromatography-tandem mass spectrometry.
10.3760/cma.j.cn121094-20210330-00177
- Author:
Bo ZHOU
1
;
Hui Ling LI
1
;
Jing MA
1
;
Fang DONG
1
;
Yan YU
1
Author Information
1. Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science, Beijing 100020, China.
- Publication Type:Journal Article
- Keywords:
Bongkrekic acid;
Chromatography, liquid;
High performance liquid chromatography-tandem mass spectrometry;
Plasma;
Tandem mass spectrometry
- MeSH:
Bongkrekic Acid;
Chromatography, High Pressure Liquid;
Humans;
Solid Phase Extraction;
Tandem Mass Spectrometry
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(3):219-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for rapid determination of bongkrekic acid (BA) in plasma by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods: In November 2020, plasma samples were extracted by methanol and acetonitrile (1∶1) and purified directly. The samples were separated by C18 column. Gradient elution was carried out with 5 mmol/L ammonium acetate water acetonitrile solution as mobile phase. Under the optimized instrument conditions, the electrospray ionization multiple reaction monitoring (MRM) mode was used, and the external standard method was used for quantitative analysis. Results: The linear relationship of BA in plasma was good in the concentration range of 2-100 μg/L, the correlation coefficient was 0.9998, the average recovery was 83.7%-112.0%, the relative standard deviation within and between batches was less than 10%, the detection limit of the method was 0.7 μg/L and the lower limit of quantification was 2.0 μg/L. Conclusion: The method is simple, rapid, accurate and sensitive, and can meet the requirements for the determination of BA in blood samples of poisoning patients.
